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Gene expression

RNA interference in adult mice

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Abstract

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

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Figure 1: RNA interference in adult mice.

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References

  1. Elbashir, S. M. et al. Nature 411, 494–498 (2001).

    Article  ADS  CAS  Google Scholar 

  2. Zhang, G., Budker, V. & Wolff, J. A. Hum. Gene Therapy 10, 1735–1737 (1999).

    Article  CAS  Google Scholar 

  3. Liu, F., Song, Y. & Liu, D. Gene Therapy 6, 1258–1266 (1999).

    Article  CAS  Google Scholar 

  4. Chang, J., Sigal, L. J., Lerro, A. & Taylor, J. J. Virol. 75, 3469–3473 (2001).

    Article  CAS  Google Scholar 

  5. Contag, C. H. et al. Photochem. Photobiol. 66, 523–531 (1997).

    Article  CAS  Google Scholar 

  6. Yant, S. R. et al. Nature Genet. 25, 35–41 (2000).

    Article  CAS  Google Scholar 

  7. Bitko, V. & Barik, S. BMC Microbiol. 1, 34 (2001).

    Article  CAS  Google Scholar 

  8. Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J. & Conklin, D. S. Genes Dev. 16, 948–958 (2002).

    Article  CAS  Google Scholar 

  9. Tuschl, T. Nature Biotechnol. 20, 446–448 (2002).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, 94305-5208, California, USA

    Anton P. McCaffrey, Leonard Meuse, Thu-Thao T. Pham & Mark A. Kay

  2. Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, 11724, New York, USA

    Douglas S. Conklin & Gregory J. Hannon

Authors
  1. Anton P. McCaffrey
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  2. Leonard Meuse
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  3. Thu-Thao T. Pham
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  4. Douglas S. Conklin
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  5. Gregory J. Hannon
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  6. Mark A. Kay
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Corresponding author

Correspondence to Mark A. Kay.

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The authors declare no competing financial interests.

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McCaffrey, A., Meuse, L., Pham, TT. et al. RNA interference in adult mice. Nature 418, 38–39 (2002). https://doi.org/10.1038/418038a

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