Abstract
NEUROGENESIS continues throughout adulthood in discrete regions. Proliferative zones include the subependymal zone1–4, from where progenitors migrate along the rostral migratory pathway to differentiate into neurons in the olfactory bulb4, and the hippocampal subgranular zone, where they migrate and differentiate into granule neurons5–7. Progenitors isolated from adult subependymal zone exhibit in vitro neurogenesis when stimulated with epidermal8,9 or fibroblast growth factor10. Cultured adult rat hippocampal progenitors (AHPs) grafted to adult rat hippocampus show site-specific neuronal differentiation11. Here we investigate determinants of multipotentiality in the adult central nervous system, by grafting AHPs into homotypic (hippocampus) or heterotypic (the rostral migratory pathway) neurogenic sites or a heterotypic, non-neurogenic site (the cerebellum). We found that grafts into neurogenic, but not non-neurogenic sites, showed neuronal differentiation. Furthermore, AHPs grafted in the rostral migratory pathway migrated into the olfactory bulb, differentiating into tyrosine-hydroxylase-positive neurons, a non-hippocampus phenotype. These results reveal that AHP populations can respond to persistent neuronal differentiation cues in the adult central nervous system.
Similar content being viewed by others
References
Gage, F. H., Ray, J. & Fisher, L. J. Annu. Rev. Neurosci. 18, 159–192 (1995).
Kaplan, M. S. & Hinds, J. W. Science 197, 1092–1094 (1977).
Levison, S. V. & Goldman, J. E. Neuron 10, 201–212 (1993).
Luskin, M. B. Neuron 11, 173–189 (1993).
Altman, J. & Das, G. D. J. Comp. Neurol. 124, 319–336 (1965).
Bayer, S. A. Exp. Brain Res. 46, 315–323 (1982).
Schwartz-Levey, M., Chikaraishi, D. M. & Kauer, J. S. J. Neurosci. 11, 3556–3564 (1991).
Reynolds, B. A. & Weiss, S. Science 255, 1707–1710 (1992).
Lois, C. & Alvarez-Buylla, A. Proc. Natl Acad. Sci. USA 90, 2074–2077 (1993).
Richards, L. J., Kilpatrick, T. J. & Bartlett, P. F. Proc. Natl Acad. Sci. USA 89, 8591–8595 (1992).
Gage, F. H. et al. Proc. Natl Acad. Sci. USA 92, 11879–11883 (1995).
Palmer, T. D., Ray, J. & Gage, F. H. Mol. Cell. Neurosci. 6, 474–486 (1995).
Menezes, J. R., Smith, C. M., Nelson, K. C. & Luskin, M. B. Mol. Cell. Neurosci. 6, 496–508 (1995).
Lois, C. & Alvarez-Buylla, A. Science 264, 1145–1148 (1994).
Lois, C., Garcia-Verdugo, J.-M. & Alvarez-Buylla, A. Science 271, 978–981 (1996).
Rousselot, P., Lois, C. & Alvarez-Buylla, A. J. Comp. Neurol. 351, 51–61 (1995).
Hu, H., Tomasiewicz, H., Magnuson, T. & Rutishauser, U. Neuron 16, 753–743 (1996).
O'Rourke, N. A. Neuron 16, 1061–1064 (1996).
Edelman, G. M. & Rutishauser, U. J. Supramol. Struct. Cell. Biochem. 16, 259–268 (1981).
Miragall, F., Kadmon, G., Husman, M. & Schachner, M. Dev. Biol. 129, 516–531 (1988).
Seki, T. & Arai, Y. Neurosci. Res. 12, 503–513 (1991).
Gao, W. Q. & Hatten, M. E. Science 260, 367–369 (1993).
Vicario-Abejon, C., Cunningham, M. G. & McKay, R. D. G. J. Neurosci. 15, 6351–6363 (1995).
Mullen, R. J., Buck, C. R. & Smith, A. M. Development 116, 201–211 (1992).
Celio, M. R. Neuroscience 35, 375–475 (1990).
Halasz, N. et al. Brain Res. 126, 455–474 (1977).
Campbell, K., Olsson, M. & Björklund, A. Neuron 15, 1259–1273 (1995).
Fishell, G. Development 121, 803–812 (1995).
Rougon, G., Dubois, C., Buckley, N., Magnani, J. L. & Zollinger, W. J. Cell Biol. 103, 2429–2437 (1986).
Keverne, E. B. Curr. Opin. Neurobiol. 5, 482–488 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Suhonen, J., Peterson, D., Ray, J. et al. Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo. Nature 383, 624–627 (1996). https://doi.org/10.1038/383624a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/383624a0
- Springer Nature Limited
This article is cited by
-
Review: adult neurogenesis contributes to hippocampal plasticity
Cell and Tissue Research (2018)
-
Analyses of distribution and dosimetry of brain metastases in small cell lung cancer with relation to the neural stem cell regions: feasibility of sparing the hippocampus in prophylactic cranial irradiation
Radiation Oncology (2017)
-
An l-Glutamine Transporter Isoform for Neurogenesis Facilitated by l-Theanine
Neurochemical Research (2017)
-
Gene Expression Profiling in the Hippocampus of Orchidectomized Rats
Journal of Molecular Neuroscience (2015)