Abstract
DURING vertebrate limb development, positional information must be specified along three distinct axes. Although much progress has been made in our understanding of the molecular interactions involved in anterior–posterior and proximal–distal limb patterning, less is known about dorsal–ventral patterning1–3. The genes Wnt-7a and Lmx-1, which are expressed in dorsal limb ectoderm and mesqderm, respectively, are thought to be important regulators of dorsal limb differentiation4–6. Whether a complementary set of molecules controls ventral limb development has not been clear. Here we report that Engrailed-1, a homeodomain-containing transcription factor expressed in embryonic ventral limb ectoderm7–8, is essential for ventral limb patterning. Loss of Engrailed-1 function in mice results in dorsal transformations of ventral paw structures, and in subtle alterations along the proximal–distal limb axis. Engrailed-1 seems to act in part by repressing dorsal differentiation induced by Wnt-7a, and is essential for proper formation of the apical ectodermal ridge.
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Johnson, R. L., Riddle, R. D. & Tabin, C. Curr. Opin. Genet. Dev. 4, 535–542 (1994).
Tickle, C. Curr. Opin. Genet Dev. 5, 478–484 (1995).
Martin, G. R. Nature 374, 410–411 (1995).
Parr, B. A. & McMahon, A. P. Nature 374, 350–353 (1995).
Riddle, R. D. et al. Cell 83, 631–640 (1995).
Vogel, A., Rodriguez, C., Warnken, W. & Izpisua Belmonte, J. C. Nature 378, 716–720 (1995).
Davis, C. A. & Joyner, A. L. Genes Dev. 2, 1736–1744 (1988).
Joyner, A. L. & Martin, G. R. Genes Dev. 1, 29–38 (1987).
MacCabe, J. A., Errick, J. & Saunders, J. W. Jr Devl Biol. 39, 69–82 (1974).
Patau, M.-P. in Vertebrate Limb and Somite Morphogenesis (eds Ede, D. A., Hinchliffe, J. R. & Balls, M.) 257–266 (Cambridge Univ. Press, 1977).
Geduspan, J. S. & MacCabe, J. A. Devl Biol. 124, 398–408 (1987).
Geduspan, J. S. & MacCabe, J. A. Anat. Rec. 224, 79–87 (1989).
Wurst, W., Auerbach, A. B. & Joyner, A. L. Development 120, 2065–2075 (1994).
Hanks, M., Wurst, W., Anson-Cartwright, L., Auerbach, A. B. & Joyner, A. L. Science 269, 679–682 (1995).
Lyons, K. M., Pelton, R. W. & Hogan, B. L. M. Development 109, 833–844 (1990).
Crossley, P. H. & Martin, G. R. Development 121, 439–451 (1995).
Parr, B. A., Shea, M. J., Vassileva, G. & McMahon, A. P. Development 119, 247–261 (1993).
Sanicola, M., Sekelsky, J., Elson, S. & Gelbart, W. M. Genetics 139, 745–756 (1995).
Zecca, M., Basler, K. & Struhl, G. Development 121, 2265–2278 (1995).
Tabata, T., Schwartz, C., Gustavson, E., Ali, Z. & Kornberg, T. B. Development 121, 3359–3369 (1995).
Lufkin, T. et al. Nature 359, 835–841 (1992).
Wilkinson, D. G., Bailes, J. A. & McMahon, A. P. Cell 50, 79–88 (1987).
Lyons, K. M., Pelton, R. W. & Hogan, B. L. M. Genes Dev. 3, 1657–1668 (1989).
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Loomis, C., Harris, E., Michaud, J. et al. The mouse Engrailed-1 gene and ventral limb patterning. Nature 382, 360–363 (1996). https://doi.org/10.1038/382360a0
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DOI: https://doi.org/10.1038/382360a0
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