Abstract
THE anterior determinant bicoid (bcd) of Drosophila is a homeo-domain protein. It forms an anterior-to-posterior gradient in the embryo and activates, in a concentration-dependent manner, several zygotic segmentation genes during blastoderm formation1–4. Its posterior counterpart, the homeodomain transcription factor caudal (cad)5–7, forms a concentration gradient in the opposite direction, emanating from evenly distributed messenger RNA in the egg. In embryos lacking bed activity as a result of mutation, the cad gradient fails to form and cad becomes evenly distributed throughout the embryo8. This suggests that bed may act in the region-specific control of cad mRNA translation. Here we report that bed binds through its homeodomain to cad mRNA in vitro, and exerts translational control through a bed-binding region of cad mRNA.
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St Johnston, R. D. & Nüsslein-Volhard, C. Cell 68, 201–219 (1992).
Pankratz, M. J. & Jäckle, H. in The Development of Drosophila melanogaster (eds Bate, M. & Martinez Arias, A.) 467–516 (Cold Spring Harbor Laboratory Press, NY, 1993).
Driever, W. & Nüsslein-Volhard, C. Cell 54, 83–93 (1988).
Struhl, G., Struhl, K. & Macdonald, P. M. Cell 57, 1259–1263 (1989).
Rivera-Pomar, R., Lu, X., Perrimon, N., Taubert, H. & Jäckle, H. Nature 376, 253–256 (1995).
Mlodzik, M., Fjose, A. & Gehring. W. J. EMBO J. 4, 2961–2969 (1985).
Macdonald, P. & Struhl, G. Nature 324, 537–545 (1986).
Mlodzik, M. & Gehring, W. J. Development 101, 421–435 (1987).
Mlodzik, M. & Gehring, W. J. Cell 48, 465–478 (1987).
Pieler, T. & Theunissen, O. Trends biocnem. Sci. 18, 226–230 (1993).
Sauer, F. & Jäckle, H. Nature 353, 563–566 (1991).
Driever, W. & Nüsslein-Volhard, C. Nature 337, 138–143 (1989).
Ruden, D. & Jäckle, H. Development 121, 63–73 (1995).
Sauer, F. & Jäckle, H. M. Nature 364, 454–457 (1993).
Lehmann, R. & Nüsslein-Volhard, C. Nature 329, 167–170 (1987).
Tautz, D. Nature 332, 281–284 (1988).
Wharton, R. & Struhl, G. Cell 67, 955–967 (1991).
Struhl, G. Nature 338, 741–744 (1989).
Hülskamp, M., Schröder, C., Pfeifle, C., Jäckle, H. & Tautz, D. Nature 338, 629–632 ((1988).
Murata, Y. & Wharton, R. Cell 80, 747–756 (1995).
Berleth, T. et al. EMBO J. 7, 1749–1756 (1988).
Driever, W. thesis, Eberhard-Karls-Univ. Tübingen (1989).
Simpson-Brose, M., Treisman, J. & Desplan, C. Cell 78, 855–865 (1994).
Tautz, D. & Pfeifle, C. Chromosoma 98, 81–85 (1989).
Rebagliati, M. Cell 58, 231–232 (1989).
Müller, M. et al. EMBO J. 7, 4299–4304 (1988).
Hess, M. A. & Duncan, R. J. biol. Chem. 269, 10913–10922 (1994).
Soeller, W., Poole, S. & Kornberg, T. Genes Dev. 2, 68–81 (1988).
Kerrigan, L. A., Croston, G. E., Lira, L. M. & Kadonaga, J. T. J. biol. Chem. 266, 574–582 (1991).
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Rivera-Pomar, R., Niessing, D., Schmidt-Ott, U. et al. RNA binding and translational suppression by bicoid. Nature 379, 746–749 (1996). https://doi.org/10.1038/379746a0
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DOI: https://doi.org/10.1038/379746a0
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