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Crystal structure of a GA protein βγdimer at 2.1 Å resolution

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An Erratum to this article was published on 29 February 1996

Abstract

MANY signalling cascades use seven-helical transmembrane receptors coupled to heterotrimeric G proteins (Gαβγ) to convert extracellular signals into intracellular responses1. Upon nucleotide exchange catalysed by activated receptors, heterotrimers dissociate into GTP-bound Gα subunits and Gβγ dimers, either of which can modulate many downstream effectors2,3. Here we use multiwavelength anomalous diffraction data to solve the crystal structure of the βγ dimer of the G protein transducin. The β-subunit is primarily a seven-bladed β-propeller that is partially encircled by an extended γ-subunit. The β-propeller, which contains seven structurally similar WD repeats, defines the stereochemistry of the WD repeat and the probable architecture of all WD-repeat-containing domains. The structure details interactions between G protein β- and γ-subunits and highlights regions implicated in effector modulation for the conserved family of G protein βγ dimers.

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References

  1. Rens-Domiano, S. & Hamm, H. E. FASEB J. 9, 1059–1066 (1995).

    Article  CAS  Google Scholar 

  2. Hepler, J. R. & Gilman, A. G. Trends biochem. Sci. 17, 383–387 (1992).

    Article  CAS  Google Scholar 

  3. Clapham, D. E. & Neer, E. J. Nature 365, 403–406 (1993).

    Article  ADS  CAS  Google Scholar 

  4. Murzin, A. G. Proteins 14, 191–201 (1992).

    Article  CAS  Google Scholar 

  5. Fong, H. K. W. et al. Proc. natn. Acad. Sci. U.S.A. 83, 2162–2166 (1996).

    Article  ADS  Google Scholar 

  6. Neer, J. E., Schmidt, C. J., Nambudripad, R. & Smith, T. F. Nature 371, 297–300 (1994).

    Article  ADS  CAS  Google Scholar 

  7. Duronio, R. J., Gordon, J. I. & Boguski, M. S. Proteins 13, 41–56 (1992).

    Article  CAS  Google Scholar 

  8. Van der Voom, L. & Ploegh, H. L. FEBS Lett. 30, 131–134 (1992).

    Article  Google Scholar 

  9. Cole, G. M. & Reed, S. I. Cell 64, 703–716 (1991).

    Article  CAS  Google Scholar 

  10. Grishin, A. V., Weiner, J. L. & Blumer, K. J. Genetics 138, 1081–1092 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Ito, N., et al. Nature 350, 87–90 (1991).

    Article  ADS  CAS  Google Scholar 

  12. Higgins, J. B. & Casey, P. J. J. biol. Chem. 269, 9067–9073 (1994).

    CAS  PubMed  Google Scholar 

  13. Lupas, A., Van Dyke, M. & Stock, J. Science 252, 1162–1164 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Garristen, A., Van Galen, P. J. M. & Simonds, W. F. Proc. natn. Acad. Sci. U.S.A. 90, 7706–7710 (1993).

    Article  ADS  Google Scholar 

  15. Ray, K., Kunsch, C., Bonner, L. M. & Robishaw, J. D. J. biol. Chem. 270, 21765–21771 (1995).

    Article  CAS  Google Scholar 

  16. Katz, A. & Simon, M. I. Proc. natn. Acad Sci. U.S.A. 92, 1998–2002 (1995).

    Article  ADS  CAS  Google Scholar 

  17. Kleuss, C. et al. Science 259, 832–834 (1993).

    Article  ADS  CAS  Google Scholar 

  18. Ueda, N. et al. J. biol. Chem. 269, 4388–4395 (1994).

    CAS  PubMed  Google Scholar 

  19. Watson, A. J., Katz, A. & Simon, M. I. J. biol. Chem. 269, 22150–22156 (1994).

    CAS  PubMed  Google Scholar 

  20. Lee, C., Murakami, T. & Simonds, W. F. J. biol. Chem. 270, 8779–8784 (1995).

    Article  CAS  Google Scholar 

  21. Garritsen, A. & Simonds, W. F. J. biol. Chem. 269, 24418–24423 (1994).

    CAS  PubMed  Google Scholar 

  22. Logothetis, D. E., Kurachi, Y. Galper, J., Neer, E. J. & Clapham, D. E. Nature 325, 321–326 (1987).

    Article  ADS  CAS  Google Scholar 

  23. Grishin, A. V., Weiner, J. L. & Blumer, K. Molec. cell. Biol. 14, 4571–4578 (1994).

    Article  CAS  Google Scholar 

  24. Leberer, E. et al. EMBO 11, 4805–4813 (1992).

    Article  CAS  Google Scholar 

  25. Lambright, D. G. et al. Nature 379, 311–319 (1996).

    Article  ADS  CAS  Google Scholar 

  26. Bigay, J. & Chabre, M. Meth. Enzym. 237, 139–146 (1994).

    Article  CAS  Google Scholar 

  27. Brunger, A. T. XPLOR Version 3.1 Manual (Yale University, Connecticut, 1993).

    Google Scholar 

  28. Jones, T. A. et al. Acta crystallogr. A47, 110–119 (1991).

    Article  CAS  Google Scholar 

  29. Carson, M. J. appl. Crystallogr. 24, 958–961 (1991).

    Article  Google Scholar 

  30. Whiteway, M. et al. Molec. cell. Biol. 14, 3223–3229 (1994).

    Article  CAS  Google Scholar 

  31. Lee, B. & Richards, F. M. J. molec. Biol. 55, 379–400 (1971).

    Article  CAS  Google Scholar 

  32. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    Article  CAS  Google Scholar 

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Author notes

  1. David G. Lambright

    Present address: Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts, 01605, USA

  2. Paul B. Sigler: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry and Howard Hughes Medical Institute, Yale University, 295 Congress Avenue, New Haven, Connecticut, 06510, USA

    John Sondek, Andrew Bohm, David G. Lambright & Paul B. Sigler

  2. Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois, 60612, USA

    Heidi E. Hamm

Authors
  1. John Sondek
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  2. Andrew Bohm
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  3. David G. Lambright
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  4. Heidi E. Hamm
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  5. Paul B. Sigler
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Sondek, J., Bohm, A., Lambright, D. et al. Crystal structure of a GA protein βγdimer at 2.1 Å resolution. Nature 379, 369–374 (1996). https://doi.org/10.1038/379369a0

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