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Structure and mechanism of DNA topoisomerase II

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An Erratum to this article was published on 14 March 1996

Abstract

The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.

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Author notes

  1. James M. Berger

    Present address: Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts, 02142, USA

  2. Steven J. Gamblin

    Present address: National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW71AA, UK

  3. Stephen C. Harrison: Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA

Authors and Affiliations

  1. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA

    James M. Berger, Steven J. Gamblin & James C. Wang

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  1. James M. Berger
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  2. Steven J. Gamblin
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  3. Stephen C. Harrison
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  4. James C. Wang
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Berger, J., Gamblin, S., Harrison, S. et al. Structure and mechanism of DNA topoisomerase II. Nature 379, 225–232 (1996). https://doi.org/10.1038/379225a0

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