Abstract
THE mechanism underlying the differentiation of CD4+ T cells into functionally distinct subsets (Thl and Th2) is incompletely understood1–3, and hitherto unidentified cytokines may be required for the functional maturation of these cells4. Here we report the cloning of a recently identified IFN-γ-inducing factor (IGIF) that augments natural killer (NK) activity in spleen cells5,6. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids, which have no obvious similarities to any peptide in the databases. Messenger RNAs for IGIF and interleukin-12 (IL-12) are readily detected in Kupffer cells and activated macrophages. Recombinant IGIF induces IFN-γ more potently than does IL-12, apparently through a separate pathway. Administration of anti-IGIF antibodies prevents liver damage in mice inoculated with Propionibacterium acnes and challenged with lipopolysaccharide, which induces toxic shock. IGIF may be involved in the development of Thl cells and also in mechanisms of tissue injury in inflammatory reactions.
Similar content being viewed by others
References
Mosmann, T. R. & Coffman, R. L. A. Rev. Immun. 7, 145–173 (1989).
Scott, P. Science 260, 496–497 (1993).
Le-Gros, G. S., Ben-Sasson, S. Z., Seder, R., Finkelman, F. D. & Paul, W. E. J. exp. Med. 172, 921–929 (1990).
Locksley, R. M. Proc. natn. Acad. Sci. U.SA. 90, 5879–5880 (1993).
Nakamura, K., Okamura, H., Nagata, K., Komatsu, T. & Tamura, T. Infect. Immun. 61, 64–70 (1993).
Okamura, H. et al. Infect. Immun. (in the press).
Ferluga, J. & Allison, A. C. Lancet 661–662 (1978).
Tsutsui, H., Mizoguchi, Y. & Morisawa, S. Hepato-gastroenterology 39, 553–559 (1992).
Wysocka, M. et al. Eur. J. Immun. 25, 672–676 (1995).
Karasuyama, H., Tohyama, N. & Tada, T. J. exp. Med. 196, 13–25 (1989).
Eyhorn, S. et al. J. Hepatol. 6, 23–35 (1988).
Chomczynski, P. & Sacchi, L. Analyt. Biochem. 162, 156–159 (1987).
Wynn, T. A., Eltoum, I., Oswald, I. P., Cheever, A. W. & Sher, A. J. Expl Med. 179, 1551–1561 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okamura, H., Tsutsui, H., Komatsu, T. et al. Cloning of a new cytokine that induces IFN-γ production by T cells. Nature 378, 88–91 (1995). https://doi.org/10.1038/378088a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/378088a0
- Springer Nature Limited
This article is cited by
-
Investigating the Potential Mechanisms and Therapeutic Targets of Inflammatory Cytokines in Post-stroke Depression
Molecular Neurobiology (2024)
-
The prognostic value and immune correlation of IL18 expression and promoter methylation in renal cell carcinoma
Clinical Epigenetics (2023)
-
Interleukin-18-primed human umbilical cord-mesenchymal stem cells achieve superior therapeutic efficacy for severe viral pneumonia via enhancing T-cell immunosuppression
Cell Death & Disease (2023)
-
Single nucleotide polymorphisms of Interleukin − 4, Interleukin-18, FCRL3 and sPLA2IIa genes and their association in pathogenesis of endometriosis
Molecular Biology Reports (2023)
-
Periodontal disease as a model to study chronic inflammation in aging
GeroScience (2023)