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Failure of B-cell differentiation in mice lacking the transcription factor EBF

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Abstract

EARLY B-cell factor (EBF) is a cell type-specific transcription factor that is expressed at all antigen-independent stages of B-lym-phocyte differentiation and participates in the regulation of the mb-1 gene1á¤-4. Here we show, by targeted gene disruption in mice, that EBF is necessary for the generation of immunoglobulin-expressing B cells. EBF-deficient mice lack B cells that have rearranged their immunoglobulin D and JH gene segments, but contain B220+CD43+ progenitor cells that express germline áµ and IL-7 receptor transcripts. Various non-lymphoid tissues that express EBF are apparently normal in homozygous mutant mice, including olfactory neurons in which EBF was identified as Olf-1 (refs 5, 6). Together, these data suggest that EBF plays a specific and important role in the transcriptional control of B-cell differentiation at a stage before Ig (immunoglobulin) gene rearrangement but after commitment of cells to the B-lymphoid lineage.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Microbiology and Immunology, University of California, San Francisco, California, 94143, USA

    Haishan Lin & Rudolf Grosschedl

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  1. Haishan Lin
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  2. Rudolf Grosschedl
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Lin, H., Grosschedl, R. Failure of B-cell differentiation in mice lacking the transcription factor EBF. Nature 376, 263–267 (1995). https://doi.org/10.1038/376263a0

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