Skip to main content
SpringerLink
Log in

Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

A NUMBER of murine T-cell hybridomas undergo apoptosis within a few hours of activation by specific antigens, mitogens, antibodies against the T-cell antigen receptor, or a combination of phorbol ester and calcium ionophore1–3. This phenomenon has been extensively studied as a model for clonal deletion in the immune system, in which potentially autoreactive T cells eliminate themselves by apoptosis after activation, either in the thymus4 or in the periphery5. Here we show that the Fas/CD95 receptor, which can transduce a potent apoptotic signal when ligated6,7, is rapidly expressed following activation of T-cell hybridomas, as is its functional, membrane-bound ligand8. Interference with the ensuing Fas/Fas-ligand interaction inhibits activation-induced apoptosis. Because T-cell receptor ligation can induce apoptosis in a single T hybridoma cell, we suggest that the Fas/Fas-ligand interaction can induce cell death in a cell-autonomous manner.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shi, Y., Sahai, B. M. & Green, D. R. Nature 339, 625–626 (1989).

    Article  ADS  CAS  Google Scholar 

  2. Ucker, D. S., Ashwell, J. D. & Nickas, G. J. Immun. 143, 3461–3469 (1989).

    CAS  Google Scholar 

  3. Shi, Y. et al. J. Immun. 144, 3326–3333 (1990).

    CAS  Google Scholar 

  4. Smith, C. A., Williams, G. T., Kingston, R., Jenkinson, E. J. & Owen, J. J. T. Nature 337, 181–184 (1989).

    Article  ADS  CAS  Google Scholar 

  5. Kawabe, Y. & Ochi, A. Nature 349, 245–248 (1991).

    Article  ADS  CAS  Google Scholar 

  6. Yonehara, S., Ashii, A. & Yonehara, M. J. exp. Med. 169, 1747–1756 (1989).

    Article  CAS  Google Scholar 

  7. Trauth, B. C. et al. Science 245, 301–305 (1989).

    Article  ADS  CAS  Google Scholar 

  8. Suda, T., Takahashi, T., Golstein, P. & Nagata, S. Cell 75, 1169–1178 (1993).

    Article  CAS  Google Scholar 

  9. Rouvier, E., Luciani, M. F. & Golstein, P. J. exp. Med. 177, 195–200 (1993).

    Article  CAS  Google Scholar 

  10. Ramsdell, F. et al. Eur. J. Immun. 24, 928–933 (1994).

    Article  CAS  Google Scholar 

  11. Crowe, P. D., VanArsdale, T. L., Walter, B. N., Dahms, K. M. & Ware, C. F. J. Immun. Meth. 168, 79–89 (1994).

    Article  CAS  Google Scholar 

  12. Ramsdell, F. et al. Int. Immun. 6, 1545–1553 (1994).

    Article  CAS  Google Scholar 

  13. Ashwell, J. D., Cunningham, R. E., Noguchi, P. D. & Hernandez, D. J. exp. Med. 165, 173–194 (1987).

    Article  CAS  Google Scholar 

  14. Green, D. R., Bissonnette, R. P., Glynn, J. M. & Shi, Y. Sem. Immun. 4, 379–388 (1992).

    CAS  Google Scholar 

  15. Mercep, M., Noguchi, P. D. & Ashwell, J. D. J. Immun. 142, 4085–4092 (1989).

    CAS  Google Scholar 

  16. Zacharchuk, C. M., Mercep, M., Chakroborti, P. K., Simons, S. S. & Ashwell, J. D. J. Immun. 145, 4037–4045 (1990).

    CAS  Google Scholar 

  17. Iwata, M., Hanaoka, S. & Sato, K. Eur. J. Immun. 21, 643–648 (1991).

    Article  CAS  Google Scholar 

  18. Iwata, M., Mukai, M., Nakai, Y. & Iseki, R. J. Immun. 149, 3302–3308 (1991).

    Google Scholar 

  19. Yang, Y., Vacchio, M. S. & Ashwell, J. D. Proc. natn. Acad. Sci. U.S.A. 90, 6170–6174 (1993).

    Article  ADS  CAS  Google Scholar 

  20. Shi, Y. et al. Science 257, 212–214 (1992).

    Article  ADS  CAS  Google Scholar 

  21. Bissonnette, R. P., McGahon, A. & Green, D. R. J. exp. Med. 180, 2413–2418 (1994).

    Article  CAS  Google Scholar 

  22. Liu, Z.-G., Smith, S. W., McLaughlin, K. A., Schwartz, L. M. & Osborne, B. A. Nature 367, 281–284 (1994).

    Article  ADS  CAS  Google Scholar 

  23. Woronicz, J. D., Calnan, B., Ngo, V. & Winoto, A. Nature 367, 277–281 (1994).

    Article  ADS  CAS  Google Scholar 

  24. Sarin, A., Adams, D. H. & Henkart, P. A. J. exp. Med. 178, 1693–1700 (1993).

    Article  CAS  Google Scholar 

  25. Shi, L. et al. Science 263, 1143–1145 (1994).

    Article  ADS  CAS  Google Scholar 

  26. Fotedar, R. et al. Molec. cell. Biol. (in the press).

  27. Russell, J. H., Rush, B., Weaver, C. & Wang, R. Proc. natn. Acad. Sci. U.S.A. 88, 4409–4413 (1993).

    Article  ADS  Google Scholar 

  28. Singer, G. G. & Abbas, A. K. Immunity 1, 365–371 (1994).

    Article  CAS  Google Scholar 

  29. Matzinger, P. J. Immun. Meth. 145, 185–192 (1991).

    Article  CAS  Google Scholar 

  30. McGahon, A. J. et al. in Cell Death (eds Schwartz, L. M. & Osborne, B. A.) (Academic, Orlando, in the press).

Download references

Author information

Authors and Affiliations

  1. Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 11149 North Torrey Pines Road, La Jolla, California, 92037, USA

    Thomas Brunner, Rona J. Mogil, Drake LaFace, Nam Jin Yoo, Artin Mahboubi, Fernando Echeverri, Seamus J. Martin & Douglas R. Green

  2. Division of Biomedical Sciences, University of California, Riverside, California, 92521, USA

    Walker R. Force & Carl F. Ware

  3. Department of Immunobiology, Immunex Research and Development Corp., Seattle, Washington, 98101, USA

    David H. Lynch

Authors
  1. Thomas Brunner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rona J. Mogil
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Drake LaFace
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nam Jin Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Artin Mahboubi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fernando Echeverri
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Seamus J. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Walker R. Force
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. David H. Lynch
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Carl F. Ware
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Douglas R. Green
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brunner, T., Mogil, R., LaFace, D. et al. Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas. Nature 373, 441–444 (1995). https://doi.org/10.1038/373441a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/373441a0

  • Springer Nature Limited

This article is cited by

Search

Navigation