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Regulation of PLC-mediated signalling in vivo by CDP-diacylglycerol synthase

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Abstract

CDP-diacylglycerol synthase (CDS) is an enzyme required for the regeneration of the signalling molecule phosphatidylinositol-4,5-bisphosphate (PtdlnsP2) from phosphatidic acid. A photo-receptor cell-specific isoform of CDS from Drosophila is a key regulator of phototransduction, a G-protein-coupled signalling cascade mediated by phospholipase C. cds mutants cannot sustain a light-activated current as a result of depletion of PtdlnsP2. Overexpression of CDS increases the amplitude of the light response, demonstrating that availability of PtdlnsP2 is a determinant in the gain of this pathway. cds mutants undergo light-dependent retinal degeneration which can be suppressed by a mutation in phospholipase C. Thus, enzymes involved in PtdlnsP2 metabolism regulate phosphoinositide-mediated signalling cascades in vivo.

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  1. Charles S. Zuker: To whom correspondence should be addressed.

Authors and Affiliations

  1. Howard Hughes Medical Institute and Departments of Biology and Neurosciences, University of California at San Diego, La Jolla, California, 92093-0649, USA

    Louisa Wu, Barbara Niemeyer, Nansi Colley, Michael Socolich & Charles S. Zuker

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  1. Louisa Wu
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  2. Barbara Niemeyer
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  3. Nansi Colley
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  4. Michael Socolich
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  5. Charles S. Zuker
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Wu, L., Niemeyer, B., Colley, N. et al. Regulation of PLC-mediated signalling in vivo by CDP-diacylglycerol synthase. Nature 373, 216–222 (1995). https://doi.org/10.1038/373216a0

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