Abstract
We have purified a nascent-polypeptide-associated complex (NAC) which prevents short ribosome-associated nascent polypeptides from inappropriate interactions with proteins in the cytosol. NAC binds nascent-polypeptide domains emerging from ribosomes unless a signal peptide is fully exposed. Depletion of cytosolic proteins (including NAC) from ribosomes carrying nascent polypeptides allows the signal recognition particle (SRP) to crosslink to polypeptides irrespective of whether or not they contain signal peptides. In the absence of cytosol, proteins lacking signal peptides can be mistranslocated into the endoplasmic reticulum in vitro, albeit with low efficiency. Readdition of NAC restores the specificity of SRP and fidelity of translocation.
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Blobel, G. Proc. natn. Acad. Sci. U.S.A. 77, 1496–1500 (1980).
Rapoport, T. A. Science 258, 931–936 (1992).
Gething, M.-J. & Sambrook, J. Nature 355, 33–45 (1992).
Hendrick, J. P. & Hartl, F.-U. A. Rev. Biochem. 62, 349–384 (1993).
Walter, P., Gilmore, R. & Blobel, G. Cell 38, 5–8 (1984).
Gilmore, R., Collins, P., Johnson, J., Kellaris, K. & Rapiejko, P. Meth. Cell Biol. 34, 223–239 (1991).
Brunner, J. A. Rev. Biochem. 62, 483–514 (1993).
Görlich, D., Kurzchalia, T. V., Wiedmann, M. & Rapoport, T. A. Meth. Cell Biol. 34, 241–262 (1991).
Luirink, J. et al. Nature 359, 741–743 (1992).
Okubo, K. et al. Nature Genet. 2, 173–179 (1992).
Kanno, M., Chalut, C. & Egly, J.-M. Gene 117, 219–228 (1992).
Walter, P., Ibrahimi, I. & Blobel, G. J. Cell Biol. 91, 545–550 (1981).
Krieg, U., Walter, P. & Johnson, A. E. Proc. natn. Acad. Sci. U.S.A. 83, 8604–8608 (1986).
Kurzchalia, T. V. et al. Nature 320, 634–636 (1986).
Walter, P. & Blobel, G. J. Cell Biol. 97, 1693–1699 (1983).
High, S., Flint, N. & Dobberstein, B. J. Cell Biol. 113, 25–34 (1991).
Wiedmann, M., Kurzchalia, T. V., Bielka, H. & Rapoport, T. A. J. Cell Biol. 104, 201–208 (1987).
Friedlander, M. & Blobel, G. Nature 318, 338–343 (1985).
Rapiejko, P. J. & Gilmore, R. J. Cell Biol. 117, 493–503 (1992).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).
Gilmore, R., Blobel, G. & Walter, P. J. Cell Biol. 95, 463–469 (1982).
Malkin, L. I. & Rich, A. J. molec. Biol. 26, 329–346 (1967).
Blobel, G. & Sabatini, D. D. J. Cell Biol. 45, 130–145 (1970).
Bernabeu, C. & Lake, J. A. Proc. natn. Acad. Sci. U.S.A. 79, 3111–3115 (1982).
Yonath, A., Leonard, K. R. & Wittmann, H. G. Science 236, 813–816 (1987).
Simon, S. M. & Blobel, G. Cell 69, 677–684 (1992).
Derman, A. I., Puziss, J. W., Bassford, P. J. & Beckwith, J. EMBO J. 12, 879–888 (1993).
Walter, P. & Blobel, G. Meth. Enzym. 96, 682–691 (1983).
Walter, P. & Blobel, G. Meth. Enzym. 96, 84–93 (1983).
Wiedmann, M., Kurzchalia, T. V., Hartmann, E. & Rapoport, T. A. Nature 328, 830–833 (1987).
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Wiedmann, B., Sakai, H., Davis, T. et al. A protein complex required for signal-sequence-specific sorting and translocation. Nature 370, 434–440 (1994). https://doi.org/10.1038/370434a0
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DOI: https://doi.org/10.1038/370434a0
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