Skip to main content
SpringerLink
Log in

A serine kinase regulates intracellular localization of splicing factors in the cell cycle

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

SMALL nuclear ribonucleoprotein particles (snRNPs) and non-snRNP splicing factors containing a serine/arginine-rich domain (SR proteins) concentrate in ‘speckles’ in the nucleus of interphase cells1. It is believed that nuclear speckles act as storage sites for splicing factors while splicing occurs on nascent transcripts2. Splicing factors redistribute in response to transcription inhibition3,4 or viral infection5, and nuclear speckles break down and reform as cells progress through mitosis6. We have now identified and cloned a kinase, SRPK1, which is regulated by the cell cycle and is specific for SR proteins; this kinase is related to a Caenorhabditis elegans kinase and to the fission yeast kinase Dskl (ref. 7). SRPK1 specifically induces the disassembly of nuclear speckles, and a high level of SRPK1 inhibits splicing in vitro. Our results indicate that SRPK1 may have a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells, and the reorganization of nuclear speckles during mitosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fu, X.-D. & Maniatis, T. Nature 343, 437–441 (1990).

    Article  ADS  CAS  Google Scholar 

  2. Spector, D. L. A. Rev. Cell Biol. 9, 265–315 (1993).

    Article  CAS  Google Scholar 

  3. Carmo-Fonseca, M. et al. J. Cell Biol. 117, 1–14 (1992).

    Article  CAS  Google Scholar 

  4. O'Keefe, R. T. et al. J. Cell Biol. 124, 249–260 (1994).

    Article  CAS  Google Scholar 

  5. Jiminez-Garca, L. F. & Spector, D. L. Cell 73, 47–59 (1993).

    Article  Google Scholar 

  6. Spector, D. L., Fu, X.-D. & Maniatis, T. EMBO J. 10, 3467–3481 (1991).

    Article  CAS  Google Scholar 

  7. Takeuchi, M. & Yanagida, M. Molec. Biol. Cell 4, 247–260 (1993).

    Article  CAS  Google Scholar 

  8. Moreno, S. & Nurse, P. Cell 61, 549–551 (1990).

    Article  CAS  Google Scholar 

  9. Peter, M. et al. Cell 61, 591–602 (1990).

    Article  CAS  Google Scholar 

  10. Ward, G. E. & Kirschner, M. W. Cell 61, 561–577 (1990).

    Article  CAS  Google Scholar 

  11. Mittnacht, S. & Weinberg, R. A. Cell 65, 381–393 (1991).

    Article  CAS  Google Scholar 

  12. Baker, S. J. et al. Molec. cell. Biol. 12, 4694–4705 (1992).

    Article  ADS  CAS  Google Scholar 

  13. Zahler, A. M. et al. Genes Dev. 6, 837–847 (1992).

    Article  CAS  Google Scholar 

  14. Zamore, P. D., Patton, J. G. & Green, M. R. Nature 355, 609–614 (1992).

    Article  ADS  CAS  Google Scholar 

  15. Woppmann, A. et al. Nucleic Acids Res. 21, 2815–2822 (1993).

    Article  CAS  Google Scholar 

  16. Li, H. & Bingham, P. M. Cell 67, 335–342 (1991).

    Article  CAS  Google Scholar 

  17. Wu, J. Y. & Maniatis, T. Cell 75, 1061–1070 (1993).

    Article  CAS  Google Scholar 

  18. Amrein, H., Hedley, M. & Maniatis, T. Cell 76, 735–746 (1994).

    Article  CAS  Google Scholar 

  19. Kohtz, J. D. et al. Nature 368, 119–124 (1994).

    Article  ADS  CAS  Google Scholar 

  20. Hanks, S. K. & Quinn, A. M. Meth. Enzym. 200, 38–62 (1991).

    Article  CAS  Google Scholar 

  21. Andrade, L. E. C. et al. Proc. natn. Acad. Sci. U.S.A. 90, 1947–1951 (1993).

    Article  ADS  CAS  Google Scholar 

  22. Fu, X.-D. Nature 365, 82–85 (1993).

    Article  ADS  CAS  Google Scholar 

  23. Kranier, A. R., Conway, G. C. & Kozak, D. Genes Dev. 4, 1158–1171 (1990).

    Article  Google Scholar 

  24. Mermoud, J. E., Cohen, P. & Lamond, A. I. Nucleic Acids Res. 20, 5263–5269 (1992).

    Article  CAS  Google Scholar 

  25. Tazi, J. et al. Nature 363, 283–286 (1993).

    Article  ADS  CAS  Google Scholar 

  26. Caceres, J. F. & Krainer, A. R. EMBO J. 12, 4715–4726 (1993).

    Article  CAS  Google Scholar 

  27. Lew, D. J., Dulic, V. & Reed, S. I. Cell 66, 1197–1212 (1991).

    Article  CAS  Google Scholar 

  28. Nakagawa, J., Kitten, G. T. & Nigg, E. A. J. Cell Sci. 94, 449–462 (1989).

    PubMed  Google Scholar 

  29. Hogner, D., Lepper, K., Seibold, G. & Jost, E. Expl Cell Res. 176, 281–296 (1988).

    Article  CAS  Google Scholar 

  30. Boyle, W. J., Van Der Geer, P. & Hunter, T. Meth. Enzym. 201, 111–149 (1991).

    Google Scholar 

Download references

Author information

Author notes

  1. Xiang-Dong Fu: To whom correspondence should be addressed.

Authors and Affiliations

  1. Division of Cellular and Molecular Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0651, USA

    Jian-Fang Gui & Xiang-Dong Fu

  2. Microchemistry Facility, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    William S. Lane

  3. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, People's Republic of China

    Jian-Fang Gui

Authors
  1. Jian-Fang Gui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William S. Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiang-Dong Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gui, JF., Lane, W. & Fu, XD. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369, 678–682 (1994). https://doi.org/10.1038/369678a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/369678a0

  • Springer Nature Limited

This article is cited by

Search

Navigation