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ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor

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Abstract

Genetic control elements are usually situated in local regions of chromatin that are hypersensitive to structural probes such as DNase I. We have reconstructed the chromatin structure of the hsp70 promoter using an in vitro nucleosome assembly system. Binding of the GAGA transcription factor on existing nucleosomes leads to nucleosome disruption, DNase I hypersensitivity at the TATA box and heat-shock elements, and rearrangement of adjacent nucleosomes. ATP hydrolysis facilitates this process, suggesting that an energy-dependent pathway is involved in chromatin remodelling.

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Author notes

  1. Peter B. Becker

    Present address: Gene Expression Programme, EMBL, Meyerhofstrasse 1, D-69117, Heidel-berg, Germany

Authors and Affiliations

  1. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Building 37, Room 4C-09, Bethesda, Maryland, 20892, USA

    Toshio Tsukiyama, Peter B. Becker & Carl Wu

Authors
  1. Toshio Tsukiyama
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  2. Peter B. Becker
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  3. Carl Wu
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Tsukiyama, T., Becker, P. & Wu, C. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor. Nature 367, 525–532 (1994). https://doi.org/10.1038/367525a0

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  • DOI: https://doi.org/10.1038/367525a0

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