Abstract
THE nuclear proto-oncoprotein Myc has been implicated in the control of cell proliferation and differentiation1–3. Myc participates in transcription4,5 and belongs to the basic–helix–loop–helix (bHLH) family of regulatory proteins2,3. Here we show that Myc interacts with TFII-I, a transcription initiation factor that activates core promoters through an initiator element (Inr)6. As previously observed for the bHLH activator USF6, Myc was found to interact cooperatively with TFII-I at both Inr and upstream E-box promoter elements. However, in this case Myc interactions with TFII-I at the Inr lead to an inhibition of transcription initiation. This inhibition is selective for a TFII-I-dependent (as opposed to TFIIA-dependent) initiation pathway and correlates with the prevention of complex formation between the TATA-binding protein TBP (TFIIDτ), TFII-I and the promoter. TBP probably interacts with Myc, but only slowly. These observations indicate that Myc has the potential to interact physically and functionally with components of the general transcription machinery.
Similar content being viewed by others
References
Cole, M. D. A. Rev Genet. 20, 361–384 (1986).
Luscher, B. & Eisenman, R. N. Genes Dev. 4, 2025–20435 (1990).
Pendergast, G. C. & Ziff, E. B. Trends genet. Sci. 8, 91–96 (1992).
Kretzner, L., Blackwood, E. M. & Eisenman, R. N. Nature 359, 426–429 (1992).
Amati, B. et al. Nature 359, 423–426 (1992).
Roy, A. L. Meisterernst, M., Pognonec, P. & Roeder, R. G. Nature 354, 245–248 (1991).
Gregor, P. D., Sawadogo, M. & Roeder, R. G. Genes Dev. 4, 1730–1740 (1990).
Pognonec, P. & Roeder, R. G. Molec. cell. Blol. 11, 5125–5136 (1991).
Murre, C., Schonleber-McCaw, P. & Baltimore, D. Cell 56, 777–783 (1989).
Murre, C. et al. Cell 58, 537–544 (1989).
Davis, R. L., Cheng, P. F., Lassar, A. B. & Weintraub, H. Cell 60, 733–746 (1990).
Jones, N. Cell 61, 9–11 (1990).
Lamb, P. & McKnight, S. L. Trends biochem. Sci. 16, 417–422 (1991).
Blackwell, T. K. et al. Science 250, 1149–1152 (1990).
Blackwood, E. & Eisenman, R. N. Science 251, 1211–1217 (1991).
Pendergast, G. C., Lawe, D. & Ziff, E. B. Cell 65, 395–407 (1991).
Roeder, R. G. Trends biochem. Sci. 16, 402–408 (1991).
Roy, A. L., Malik, S., Meisterernst, M. & Roeder, R. G. Nature 365, 355–359 (1993).
Meisterernst, M., Roy, A. L., Lieu, H. L. & Roeder, R. G. Cell 66, 981–993 (1991).
Sawadogo, M. & Roeder, R. G. Cell 43, 165–175 (1985).
Blackwood, E. M., Luscher, B. & Eisenman, R. N. Genes Dev. 6, 71–80 (1992).
Wenzel, A. et al. EMBO J. 10, 3703–3712 (1991).
Rustgi, A. K., Dyson, N. & Bernards, R. Nature 352, 541–544 (1991).
Van Antwerp, M. E. et al. Proc. natn. Acad. Sci. U.S.A. 89, 9010–9014 (1992).
Bernards, R., Dessain, S. K. & Weinberg, R. A. Cell 47, 667–674 (1986).
Inghirami, G. et al. Science 250, 682–686 (1990).
Prendergast, G. C. & Cole, M. D. Molec. cell Biol. 9, 124–134 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roy, A., Carruthers, C., Gutjahr, T. et al. Direct role for Myc in transcription initiation mediated by interactions with TFII-I. Nature 365, 359–361 (1993). https://doi.org/10.1038/365359a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/365359a0
- Springer Nature Limited
This article is cited by
-
miR-320b suppresses cell proliferation by targeting c-Myc in human colorectal cancer cells
BMC Cancer (2015)
-
Decrease expression of microRNA-744 promotes cell proliferation by targeting c-Myc in human hepatocellular carcinoma
Cancer Cell International (2014)
-
Transcriptional regulation and transformation by Myc proteins
Nature Reviews Molecular Cell Biology (2005)
-
Myc represses transcription through recruitment of DNA methyltransferase corepressor
The EMBO Journal (2005)
-
Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: potential targeting for therapeutic intervention
Leukemia (2003)