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Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast

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Abstract

A novel genetic selection in yeast has been used to isolate a complementary DNA for the transcriptional activator, Olf-1, which binds to the regulatory sequences of several olfactory-specific genes. The Olf-1 protein, expressed exclusively in the olfactory receptor neurons and their precursors, contains a new helix–loop–helix motif and functions as an apparent homodimer. Olf-1 may be the first member of a family of related proteins that may direct cellular differentiation in a variety of neuronal tissues.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205, USA

    Michael M. Wang & Randall R. Reed

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  1. Michael M. Wang
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  2. Randall R. Reed
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Wang, M., Reed, R. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast. Nature 364, 121–126 (1993). https://doi.org/10.1038/364121a0

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  • DOI: https://doi.org/10.1038/364121a0

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