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Neurotrophins promote motor neuron survival and are present in embryonic limb bud

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Abstract

Embryonic spinal motor neurons are thought to depend for survival on unidentified factors secreted both by their peripheral targets and by cells within the central nervous system1. The neurotrophins are a family of polypeptides required for survival of discrete central and peripheral neuronal populations in vivo and in vitro2,3. In spite of their ability to reduce motor neuron death in vivo4–6, the known neurotrophins have been thought to be without direct effect on motor neurons7–10. Here we show that picomolar concentrations of three of them, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-5, can prevent the death of cultured embryonic rat spinal motor neurons. Furthermore, messenger RNA coding for neurotrophins is present at appropriate stages in spinal cord and limb bud, and mRNA for their receptors is found in motor neurons. These neurotrophins may therefore be physiological motor neuron growth factors.

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References

  1. Oppenheim, R. W. Trends Neurosci. 12, 252–255 (1989).

    Article  CAS  Google Scholar 

  2. Levi-Montalcini, R. Science 237, 1154–1162 (1987).

    Article  ADS  CAS  Google Scholar 

  3. Barde, Y.-A. Neuron 2, 1525–1534 (1989).

    Article  CAS  Google Scholar 

  4. Yan, Q., Elliott, J. & Snider, W. D. Nature 360, 753–755 (1992).

    Article  ADS  CAS  Google Scholar 

  5. Oppenheim, R. W., Qin-Wei, Y., Prevette, D. & Yan, Q. Nature 360, 755–757 (1992).

    Article  ADS  CAS  Google Scholar 

  6. Sendtner, M., Holtmann, B., Kolbeck, R., Thoenen, H. & Barde, Y.-A. Nature 360, 757–759 (1992).

    Article  ADS  CAS  Google Scholar 

  7. Thoenen, H. Trends Neurosci. 14, 165–170 (1991).

    Article  CAS  Google Scholar 

  8. Arakawa, Y., Sendtner, M. & Thoenen, H. J. Neurosci. 10, 3507–3515 (1990).

    Article  CAS  Google Scholar 

  9. Bloch-Gallego, E. et al. Development 111, 221–232 (1991).

    CAS  PubMed  Google Scholar 

  10. Berkemeier, L. R. et al. Neuron 7, 857–866 (1991).

    Article  CAS  Google Scholar 

  11. Camu, W. & Henderson, C. E. J. Neurosci. Meth. 44, 59–70 (1992).

    Article  CAS  Google Scholar 

  12. Hynes, M., Gitt, M., Barondes, S. H., Jessell, T. & Buck, L. B. J. Neurosci. 10, 1004–1013 (1990).

    Article  CAS  Google Scholar 

  13. Ericson, J., Thor, S., Edlund, T., Jessell, T. M. & Yamada, T. Science 256, 1555–1560 (1992).

    Article  ADS  CAS  Google Scholar 

  14. Martinou, J.-C., Martinou, I. & Kato, A. C. Neuron 8, 737–744 (1992).

    Article  CAS  Google Scholar 

  15. Lamballe, F., Klein, R. & Barbacid, M. Cell 66, 967–979 (1991).

    Article  CAS  Google Scholar 

  16. Soppet, D. et al. Cell 65, 895–903 (1991).

    Article  CAS  Google Scholar 

  17. Squinto, S. P. et al. Cell 65, 885–893 (1991).

    Article  CAS  Google Scholar 

  18. Klein, R. S. et al. Development 109, 845–850 (1990).

    CAS  PubMed  Google Scholar 

  19. Schecterson, L. C. & Bothwell, M. Neuron 9, 449–463 (1992).

    Article  CAS  Google Scholar 

  20. Martin-Zanca, D., Barbacid, M. & Parada, L. Genes Dev. 4, 683–694 (1990).

    Article  CAS  Google Scholar 

  21. Klein, R. et al. EMBO J. 8, 3701–3709 (1989).

    Article  CAS  Google Scholar 

  22. DiStefano, P. S. et al. Neuron 8, 983–993 (1992).

    Article  CAS  Google Scholar 

  23. Maisonpierre, P. C. et al. Neuron 5, 501–509 (1990).

    Article  CAS  Google Scholar 

  24. Ernfors, P. & Persson, H. Eur. J. Neurosci. 3, 953–967 (1991).

    Article  Google Scholar 

  25. Rassendren, F. A., Bloch-Gallego, E., Tanaka, H. & Henderson, C. E. Proc. natn. Acad. Sci. U.S.A. 89, 7194–7198 (1992).

    Article  ADS  CAS  Google Scholar 

  26. Martinou, J. C., Le Van Thai, A., Cassar, G., Roubinet, F. & Weber, M. J. Devl Brain Res. 52, 175–181 (1990).

    Article  CAS  Google Scholar 

  27. Vogel, K. S. & Davies, A. M. Neuron 7, 819–830 (1991).

    Article  CAS  Google Scholar 

  28. Fonnum, F. J. Neurochem. 24, 407–409 (1975).

    Article  CAS  Google Scholar 

  29. Kawasaki, S. E. & Wang, M. K. in PCR Technology: Principles and Applications for DNA Amplification (ed. Erlich, H. A.) 89–97 (Stockton, New York, 1989).

    Book  Google Scholar 

  30. Phillips, H. S. et al. Science 250, 290–294 (1990).

    Article  ADS  CAS  Google Scholar 

  31. Baranowski, A. P., Anand, U. & McMahon, S. B. Neurosci. Lett. 141, 53–56 (1992).

    Article  CAS  Google Scholar 

  32. Rosenthal, A. et al. Neuron 4, 767–773 (1990).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Centre de Recherche de Biochimie Macromoléculaire, UPR 9008 du CNRS/U249 de I'lNSERM, BP. 5051, 34033, Montpellier, France

    Christopher E. Henderson, William Camu, Clément Mettling & Annie Gouin

  2. Department of Cell Analysis and § Department of Neuroscience, Genentech Inc., South San Francisco, California, 94080, USA

    Kristian Poulsen, Mona Karihaloo, Janette Ruilamas & Tony Evans

  3. Department of Neuroscience, Genentech Inc., South San Francisco, California, 94080, USA

    Mark P. Armanini, Lucy Berkemeier, Heidi S. Phillips & Arnon Rosenthal

  4. Department of Physiology, UMDS (St Thomas' Campus), Lambeth Palace Road, London, SE1 7EH, UK

    Stephen B. McMahon

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  1. Christopher E. Henderson
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  2. William Camu
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  3. Clément Mettling
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  4. Annie Gouin
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  5. Kristian Poulsen
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  6. Mona Karihaloo
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  7. Janette Ruilamas
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  8. Tony Evans
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  9. Stephen B. McMahon
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  10. Mark P. Armanini
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  11. Lucy Berkemeier
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Henderson, C., Camu, W., Mettling, C. et al. Neurotrophins promote motor neuron survival and are present in embryonic limb bud. Nature 363, 266–270 (1993). https://doi.org/10.1038/363266a0

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  • DOI: https://doi.org/10.1038/363266a0

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