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Cloning and expression of a rat brain L-glutamate transporter

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An Erratum to this article was published on 31 December 1992

Abstract

SYNAPTIC transmission of most vertebrate synapses is thought to be terminated by rapid transport of the neurotransmitter into presynaptic nerve terminals or neuroglia1–5. L-Glutamate is the major excitatory transmitter in brain and its transport represents the mechanism by which it is removed from the synaptic cleft and kept below toxic levels5,6. Here we use an antibody against a glial L-glutamate transporter from rat brain7 to isolate a complemen-tary DNA clone encoding this transporter. Expression of this cDNA in transfected HeLa cells indicates that L-glutamate accumulation requires external sodium and internal potassium and transport shows the expected stereospecificity. The cDNA sequence predicts a protein of 573 amino acids with 8–9 putative transmembrane α-helices. Database searches indicate that this protein is not homologous to any identified protein of mammalian origin, including the recently described superfamily of neurotransmitter transporters. This protein therefore seems to be a member of a new family of transport molecules.

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References

  1. Iversen, L. L. Br. med. Bull. 29, 130–136 (1973).

    Article  CAS  Google Scholar 

  2. Bennett, J. P. Jr, Mulder, A. H. & Snyder, S. M. Life Sci. 15, 1045–1056 (1974).

    Article  CAS  Google Scholar 

  3. Balcar, V. J. & Johnston, G. A. R. J. Neurobiol. 3, 295–301 (1972).

    Article  CAS  Google Scholar 

  4. Kanner, B. I. & Schuldiner, S. CRC crit. Rev. Biochem. 22, 1–38 (1987).

    Article  CAS  Google Scholar 

  5. Nicolls, D. & Attwell, D. Trends Pharmac. Sci. 11, 462–468 (1990).

    Article  Google Scholar 

  6. McBean, G. J. & Roberts, P. J. J. Neurochem. 44, 247–254 (1985).

    Article  CAS  Google Scholar 

  7. Danbolt, N. C., Storm-Mathisen, J. & Kanner, B. I. Neuroscience 51, 295–310 (1992).

    Article  CAS  Google Scholar 

  8. Keinanen, K. et al. Science 249, 556–560 (1990).

    Article  CAS  ADS  Google Scholar 

  9. Danbolt, N. C., Pines, G. & Kanner, B. I. Biochemistry 29, 6734–6740 (1990).

    Article  CAS  Google Scholar 

  10. Guastella, J. et al. Science 249, 1303–1306 (1990).

    Article  CAS  ADS  Google Scholar 

  11. Snutch, T. P., Leonard, J. P., Gilbert, M. M., Lester, H. A. & Davidson, N. Proc. natn. Acad. Sci. U.S.A. 87, 3391–3395 (1990).

    Article  CAS  ADS  Google Scholar 

  12. Fuerst, T. R., Niles, E. G., Studier, F. W. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 83, 8122–8126 (1986).

    Article  CAS  ADS  Google Scholar 

  13. Blakely, R. D., Clark, J. A., Rudnick, G. & Amara, S. G. Analyt. Biochem. 194, 302–308 (1991).

    Article  CAS  Google Scholar 

  14. Keynan, S., Sun, Y.-J., Kanner, B. I. & Rudnick, G. Biochemistry 31, 1974–1979 (1992).

    Article  CAS  Google Scholar 

  15. Kanner, B. I. & Sharon, I. Biochemistry 17, 3949–3953 (1978).

    Article  CAS  Google Scholar 

  16. Kanner, B. I. & Bendahan, A. Biochemistry 21, 6327–6330 (1982).

    Article  CAS  Google Scholar 

  17. Pines, G. & Kanner, B. I. Biochemistry 29, 11209–11214 (1990).

    Article  CAS  Google Scholar 

  18. Bridges, R. J., Stanley, M. S., Anderson, M. W., Cotman, C. W. & Chamberlin, A. R. J. med. Chem. 34, 717–725 (1991).

    Article  CAS  Google Scholar 

  19. Ferkany, J. & Coyle, J. T. J. Neurosci. Res. 16, 491–503 (1986).

    Article  CAS  Google Scholar 

  20. Fletcher, E. & Johnston, G. A. R. J. Neurochem. 57, 911–914 (1991).

    Article  CAS  Google Scholar 

  21. Perbal, B. A Practical Guide to Molecular Cloning 2nd edn (Wiley, New York, 1988).

    Google Scholar 

  22. Kozak, M. Nucleic Acid Res. 15, 8125–8148 (1987).

    Article  CAS  Google Scholar 

  23. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  24. von Heijne, G. Eur. J. Biochem. 133, 17–21 (1983).

    Article  CAS  Google Scholar 

  25. Uhl, G. R. Trends Neurosci. 15, 265–268 (1992).

    Article  CAS  Google Scholar 

  26. Taxt, T. & Storm-Mathisen, J. Neuroscience 11, 79–100 (1984).

    Article  CAS  Google Scholar 

  27. Wilkin, G. P., Garthwaite, J. & Balázs, R. Brain Res. 244, 69–80 (1982).

    Article  CAS  Google Scholar 

  28. Tolner, B., Poolman, B., Wallace, B. & Konings, W. N. J. Bact. 174, 2391–2393 (1992).

    Article  CAS  Google Scholar 

  29. Rothstein, J. D., Martin, L. J. & Kuncl, R. W. New Engl. J. Med. 326, 1464–1468 (1992).

    Article  CAS  Google Scholar 

  30. Radian, R. & Kanner, B. I. J. biol. Chem. 260, 11859–11865 (1985).

    CAS  PubMed  Google Scholar 

  31. Cleveland, D. W. et al. Cell 20, 95–105 (1980).

    Article  CAS  Google Scholar 

  32. Sambrook, J., Fritsh, E. F. & Maniatis, T. Molecular Cloning (eds Ford, N., Natan, C. & Ferguson, M.) 2nd edn (Cold Spring Harbor Laboratory Press, New York. 1989).

    Google Scholar 

  33. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  CAS  ADS  Google Scholar 

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Author notes

  1. Baruch I. Kanner: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Biochemistry, Hadassah Medical School, The Hebrew University, PO Box 1172, 91010, Jerusalem, Israel

    Gilia Pines, Yumin Zhang, Annie Bendahan & Baruch I. Kanner

  2. Anatomical Institute, University of Oslo, PO Box 1105, Blindern, N-0137, Oslo, Norway

    Niels C. Danbolt & Jon Storm-Mathisen

  3. Biotechnology Centre, University of Oslo, PO Box 1125, Blindern, N-0136, Oslo, Norway

    Magnar Bjørås, Lars Eide & Erling Seeberg

  4. Division for Environmental Toxicology, Norwegian Defence Research Establishment, PO Box 25, N-2007, Kjeller, Norway

    Magnar Bjørås, Lars Eide & Erling Seeberg

  5. Max-Planck-Institut für Biophysik, Kennedy Allee 70, 6000, Frankfurt-am-Main, 70, Germany

    Hermann Koepsell

Authors
  1. Gilia Pines
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  2. Niels C. Danbolt
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  3. Magnar Bjørås
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  4. Yumin Zhang
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  5. Annie Bendahan
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  6. Lars Eide
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  7. Hermann Koepsell
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  8. Jon Storm-Mathisen
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  9. Erling Seeberg
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  10. Baruch I. Kanner
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Pines, G., Danbolt, N., Bjørås, M. et al. Cloning and expression of a rat brain L-glutamate transporter. Nature 360, 464–467 (1992). https://doi.org/10.1038/360464a0

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