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Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentation

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Abstract

MAJOR histocompatibility complex (MHC) class I molecules bind and deliver peptides derived from endogenously synthesized proteins to the cell surface for survey by cytotoxic T lymphocytes. It is believed that endogenous antigens are generally degraded in the cytosol, the resulting peptides being translocated into the endoplasmic reticulum where they bind to MHC class I molecules. Transporters containing an ATP-binding cassette encoded by the MHC class II region seem to be responsible for this transport1–8. Genes coding for two subunits of the '20S' proteasome (a multicatalytic proteinase) have been found in the vicinity of the two transporter genes in the MHC class II region, indicating that the proteasome could be the unknown proteolytic entity in the cytosol involved in the generation of MHC class I-binding peptides9–13. By introducing rat genes encoding the MHC-linked transporters into a human cell line lacking both transporter and proteasome subunit genes, we show here that the MHC-encoded proteasome subunits are not essential for stable MHC class I surface expression, or for processing and presentation of antigenic peptides from influenza virus and an intracellular protein.

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Authors and Affiliations

  1. Tumor Immunology Program, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany

    Frank Momburg, Vianney Ortiz-Navarrete, Jacques Neefjes & Günter J. Hämmerling

  2. Department of Immunohaematology and Blood Bank, University Hospital, Bldg 1 E3-Q, PO Box 9600, 2300 RC, Leiden, The Netherlands

    Els Goulmy & Yvonne van de Wal

  3. DNAX Research Institute, 901 California Avenue, Palo Alto, California, 94304, USA

    Hergen Spits

  4. Department of Immunology, Agricultural and Food Research Council, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, CB2 4AT, UK

    Simon J. Powis, Geoffrey W. Butcher & Jonathan C. Howard

  5. Max-Planck Institute for Biology, Corrensstrasse 42, 7400, Tübingen, Germany

    Peter Walden

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  1. Frank Momburg
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  2. Vianney Ortiz-Navarrete
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  3. Jacques Neefjes
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  4. Els Goulmy
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  6. Hergen Spits
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  7. Simon J. Powis
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  8. Geoffrey W. Butcher
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  9. Jonathan C. Howard
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  10. Peter Walden
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  11. Günter J. Hämmerling
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Momburg, F., Ortiz-Navarrete, V., Neefjes, J. et al. Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentation. Nature 360, 174–177 (1992). https://doi.org/10.1038/360174a0

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  • DOI: https://doi.org/10.1038/360174a0

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