Skip to main content
SpringerLink
Log in

Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

EWING'S sarcoma and related subtypes of primitive neuroectodermal tumours share a recurrent and specific t(ll;22) (q24;q12) chromosome translocation1–8, the breakpoints of which have recently been cloned9. Phylogenetically conserved restriction fragments in the vicinity of EWSR1 and EWSR2, the genomic regions where the breakpoints of chromosome 22 and chromosome 11 are, respectively, have allowed identification of transcribed sequences from these regions and has indicated that a hybrid transcript might be generated by the translocation9. Here we use these fragments to screen human complementary DNA libraries to show that the translocation alters the open reading frame of an expressed gene on chromosome 22 gene by substituting a sequence encoding a putative RNA-binding domain for that of the DNA-binding domain of the human homologue of murine Fli-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aurias, A., Rimbaut, C., Buffe, C., Dubousset, J. & Mazabraud, A. N. Engl. J. Med. 309, 496–497 (1983).

    Google Scholar 

  2. Turc-Carel, C., Philip, I., Berge, R. M. P., Philip, T. & Lenoir, G. M. N. Engl. J. Med. 309, 497–498 (1983).

    Google Scholar 

  3. Whang-Peng, J. et al. N. Engl. J. Med. 311, 584–585 (1984).

    Article  CAS  Google Scholar 

  4. De Chadarevian, J. P., Vekemans, M. & Seemayer, T. A. N. Engl. J. Med. 311, 1702–1703 (1984).

    CAS  PubMed  Google Scholar 

  5. Vigfusson, N. V., Allen, L. J., Phillips, J. H., Alschibaja, T. & Riches, W. G. Cancer Genet. Cytogenet. 22, 211–218 (1986).

    Article  CAS  Google Scholar 

  6. Cavazzana, A. O. et al. Adv. Neuroblastoma Res. 2, 463–473 (1988).

    Google Scholar 

  7. Whang-Peng, J., Freter, C. E., Knutsen, T., Nanfro, J. J. & Gazdar, A. Cancer Genet. Cytogenet. 29, 155–157 (1987).

    Article  CAS  Google Scholar 

  8. Turc-Carel, C. et al. Cancer Genet. Cytogenet. 32, 229–238 (1988).

    Article  CAS  Google Scholar 

  9. Zucman, J. et al. Genes Chrom. Cancer (in the press).

  10. Corden, J. L. Trends biochem. Sci. 15, 383–387 (1990).

    Article  CAS  Google Scholar 

  11. Suzuki, M. Nature 344, 562–565 (1991).

    Article  ADS  Google Scholar 

  12. Frankel, A. D., Mattaj, I. W. & Rio, D. C. Cell 67, 1041–1046 (1991).

    Article  CAS  Google Scholar 

  13. Kumar, A. et al. J. biol. Chem 265, 17094–17100 (1990).

    CAS  PubMed  Google Scholar 

  14. Munroe, S. H. & Dong, X. Proc. natn. Acad. Sci. U.S.A. 89, 895–899 (1992).

    Article  ADS  CAS  Google Scholar 

  15. Kenan, D. J., Query, C. C. & Keene, J. D. Trends biochem. Sci. 16, 214–220 (1991).

    Article  CAS  Google Scholar 

  16. Bandziulis, R. J., Swanson, M. S. & Dreyfuss, G. Genes Dev. 3, 431–437 (1989).

    Article  CAS  Google Scholar 

  17. Haynes, S. R., Rebbert, M. L., Mozer, B. A., Forquignon, F. & Dawid, I. B. Proc. natn. Acad. Sci. U.S.A. 84, 1819–1823 (1987).

    Article  ADS  CAS  Google Scholar 

  18. Ben-David, Y., Giddens, E. B., Letwin, K. & Bernstein, A. Genes Dev. 5, 908–918 (1991).

    Article  CAS  Google Scholar 

  19. Baud, V., Lipinski, M., Rassart, E., Poliqiun, L. & Bergeron, D. Genomics 11, 223–224 (1991).

    Article  CAS  Google Scholar 

  20. Turc-Carel, C., Philip, I., Berger, M. P., Philip, T. & Lenoir, G. M. Cancer Genet. Cytogenet. 12, 1–19 (1984).

    Article  CAS  Google Scholar 

  21. Douglass, E. C., Valentine, M., Green, A. A., Hayes, F. A. & Thompson, E. I. J. natn. Cancer Inst. 77, 1211–1213 (1986).

    CAS  Google Scholar 

  22. Watson, D. K., Ascione, R. & Papas, T. S. Crit. Rev. Oncogenesis 1, 409–436 (1990).

    CAS  PubMed  Google Scholar 

  23. Ben-David, Y. & Bernstein, A. Cell 66, 831–834 (1991).

    Article  CAS  Google Scholar 

  24. Mitchell, P. M. & Tjian, R. Science 245, 371–378 (1989).

    Article  ADS  CAS  Google Scholar 

  25. Turc-Carel, C., Dal Cin, P., Rao, U., Karakousis, C. & Sandberg, A. Cancer Genet. Cytogenet. 30, 145–150 (1988).

    Article  CAS  Google Scholar 

  26. Shen, W. P. V. et al. Cancer Genet. Cytogenet. 45, 207–217 (1990).

    Article  CAS  Google Scholar 

  27. Trent, J. M., Kaneko, Y. & Mitelman, F. Cytogenet. Cell Genet. 51, 533–562 (1989).

    Article  CAS  Google Scholar 

  28. Rouleau, G. A. et al. Am. J. hum. Genet. 46, 323–328 (1990).

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Kozak, M. Cell 44, 283–292 (1986).

    Article  CAS  Google Scholar 

  30. Srivastava, M., McBride, O. W., Fleming, P. J., Pollard, H. B. & Burns, A. L. J. biol Chem. 265, 14922–14931 (1990).

    CAS  PubMed  Google Scholar 

  31. Spritz, R. A. et al. Nucleic Acids Res. 15, 10173–10391 (1987).

    Article  Google Scholar 

  32. Biamonti, G. et al. J. mol. Biol. 207, 491–503 (1988).

    Article  Google Scholar 

  33. Burd, C. G., Swanson, M. S., Goerlach, M. & Dreyfuss, G. Proc. natn. Acad. Sci. U.S.A. 86, 9788–9792 (1989).

    Article  ADS  CAS  Google Scholar 

  34. Grange, T., Martin de sa, C., Oddos, J. & Pictet, R. Nucleic Acids Res. 15, 4771–4787 (1987).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Heinrich Kovar: Children's Cancer Research Institute, St Anna Kinderspital A-1090 Vienna, Austria

  2. Pieter de Jong: Human Genome Center, Lawrence Livermore National Laboratory, University of California, PO Box 5507, Livermore, California 94550, USA

  3. Guy Rouleau: Centre de Recherche en Neurosciences, McGill University, 1650 Cedar Avenue, Montreal, Quebec H3G1A4, Canada

Authors and Affiliations

  1. Laboratoires de Génétique des Tumeurs and LIRA 620 CNRS, Institut Curie, 26 rue d'Ulm, 75231, Paris, Cedex 05, France

    Olivier Delattre, Jessica Zucman, Béatrice Plougastel, Chantal Desmaze, Thomas Melot, Martine Peter, Heinrich Kovar, Isabelle Joubert, Pieter de Jong, Guy Rouleau, Alain Aurias & Gilles Thomas

Authors
  1. Olivier Delattre
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jessica Zucman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Béatrice Plougastel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chantal Desmaze
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Thomas Melot
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Martine Peter
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Heinrich Kovar
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Isabelle Joubert
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Pieter de Jong
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Guy Rouleau
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Alain Aurias
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Gilles Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Delattre, O., Zucman, J., Plougastel, B. et al. Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature 359, 162–165 (1992). https://doi.org/10.1038/359162a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/359162a0

  • Springer Nature Limited

This article is cited by

Search

Navigation