The advent of 33P nucleotide triphosphates has widened the options available for labelling nucleic acids. Choosing the most appropriate label requires a careful consideration of experimental needs and circumstances.
References
Feinberg, A. P. & Vogelstein, B. Analyt. biochem. 132, 6–13 (1983).
Rigby, P. W. J. et al. J. molec. Biol. 113, 237 (1977).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Biggin, M. D. et al. Proc. natn. Acad. Sci. U.S.A. 80, 3963–3965 (1983).
Murray, V. Nucleic Acids Res. 17, 8889 (1989).
Richardson, C. C. The Enzymes: Nucleic Acids Part A Vol. XIV (ed. Boyer, P. D.) 229 (Academic, Florida, 1981).
Orita, M. et al. Genomics 5, 874–879 (1989).
Durrant, I. Nature 346, 297–298 (1990).
Durrant, I. Biotechniques 8, 564–570 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evans, M., Read, C. 32p 33p and 35S: selecting a label for nucleic acid analysis. Nature 358, 520–521 (1992). https://doi.org/10.1038/358520a0
Issue Date:
DOI: https://doi.org/10.1038/358520a0
- Springer Nature Limited
This article is cited by
-
Evaluation of novel formulations of 35S- and 33P-labelled nucleotides for in situ hybridization
The Histochemical Journal (1995)
-
Improved procedure for differential display of transcripts from cotton tissues
Plant Molecular Biology Reporter (1995)