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Molecular and biological characterization of a murine ligand for CD40

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Abstract

THE CD40 surface molecule is a 277-amino-acid glycoprotein expressed on B lymphocytes, epithelial cells and some carcinoma cell lines. Monoclonal antibodies against CD40 mediate a variety of effects on B lymphocytes, including induction of intercellular adhesion1,2, short- and long-term proliferation3–5, differentiation6,7 and enhanced tyrosine phosphorylation of proteins8. In addition, germinal centre centrocytes are prevented from undergoing apop-tosis by activation through CD40 and receptor for antigen9. These data indicate that CD40 could be a receptor for an unknown ligand with important functions in B-cell development and activation. This hypothesis is strengthened by the homology of the extracellular region of the CD40 molecule10with a family of cell-surface glycoproteins11,12that includes the receptors for nerve growth factor13,14 and tumour necrosis factor11,15,16. Here we report the cloning of a ligand for CD40 that is expressed on the cell surface of activated T cells and mediates B-cell proliferation in the absence of co-stimulus, as well as IgE production in the presence of interIeukin-4.

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References

  1. Valle, A. et al. Eur. J. Immun. 19, 1463–1467 (1989).

    Article  CAS  Google Scholar 

  2. Barrett, T. B., Shu, G. & Clark, E. A. J. Immun. 146, 1722–1729 (1991).

    CAS  PubMed  Google Scholar 

  3. Clark, E. A. & Ledbetter, J. A. Proc. natn. Acad. Sci. U.S.A. 83, 4494–4498 (1986).

    Article  ADS  CAS  Google Scholar 

  4. Paulie, S. et al. J. Immun. 142, 590–595 (1989).

    CAS  PubMed  Google Scholar 

  5. Banchereau, J., de Paoli, P., Valle, A., Garcia, E. & Rousset, F. Science 251, 70–72 (1991).

    Article  ADS  CAS  Google Scholar 

  6. Jabara, H. H., Fu, S. M., Geha, R. S. & Vercelli, D. J. exp. Med. 172, 1861–1864 (1990).

    Article  CAS  Google Scholar 

  7. Zhang, K., Clark, E. A. & Saxon, A. J. Immun. 146, 1836–1842 (1991).

    CAS  PubMed  Google Scholar 

  8. Uckun, F. M. et al. J. biol. Chem. 266, 17478–17485 (1991).

    CAS  PubMed  Google Scholar 

  9. Liu, Y. J. et al. Nature 342, 929–931 (1989).

    Article  ADS  CAS  Google Scholar 

  10. Stamenkovic, I., Clark, E. A. & Seed, B. EMBO J. 8, 1403–1410 (1989).

    Article  CAS  Google Scholar 

  11. Smith, C. A. et al. Science 248, 1019–1023 (1990).

    Article  ADS  CAS  Google Scholar 

  12. Smith, C. A. et al. Biochem. biophys. Res. Commun. 176, 335–342 (1991).

    Article  CAS  Google Scholar 

  13. Johnson, D. et al. Cell 47, 545–554 (1986).

    Article  CAS  Google Scholar 

  14. Radeke, M. J., Misko, T. P., Hsu, C., Herzenberg, L. A. & Shooter, E. M. Nature 325, 593–597 (1987).

    Article  ADS  CAS  Google Scholar 

  15. Loetscher, H. et al. Cell 61, 351–359 (1990).

    Article  CAS  Google Scholar 

  16. Schall, T. J. et al. Cell 61, 361–370 (1990).

    Article  CAS  Google Scholar 

  17. Fanslow, W. C. et al. J. Immun. (in the press).

  18. McMahan, C. J. et al. EMBO J. 10, 2821–2832 (1991).

    Article  CAS  Google Scholar 

  19. Kriegler, M., Perez, C., DeFay, K., Albert, I. & Lu, S. D. Cell 53, 45–53 (1988).

    Article  CAS  Google Scholar 

  20. Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).

    Article  CAS  Google Scholar 

  21. Coffman, R. L. et al. Immunol. Rev. 102, 5–28 (1988).

    Article  CAS  Google Scholar 

  22. Leo, O., Foo, M., Sachs, D. H., Samelson, L. E. & Bluestone, J. A. Proc. natn. Acad. Sci. U.S.A. 84, 1374–1378 (1987).

    Article  ADS  CAS  Google Scholar 

  23. Spriggs, M. K. et al. J. biol. Chem. 265, 22499–22505 (1990).

    CAS  PubMed  Google Scholar 

  24. Braesch-Anderson, S. et al. J. Immun. 142, 562–567 (1989).

    Google Scholar 

  25. Thomas, G., Thorne, B. A. & Hruby, D. E. A. Rev. Physiol. 50, 323–332 (1988).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Immunology, Immunex Research & Development Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Richard J. Armitage, William C. Fanslow, Timothy A. Sato, Ky N. Clifford, Brian M. Macduff, Charles R. Maliszewski & Kenneth H. Grabstein

  2. Department of Molecular Biology, Immunex Research & Development Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Laura Strockbine, Dirk M. Anderson, Steven D. Gimpel, David Cosman & Melanie K. Spriggs

  3. Department of Biochemistry, Immunex Research & Development Corporation, 51 University Street, Seattle, Washington, 98101, USA

    Terri Davis-Smith & Craig A. Smith

  4. Department of Microbiology SC-42, University of Washington, Seattle, Washington, 98195, USA

    Edward A. Clark

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  1. Richard J. Armitage
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  2. William C. Fanslow
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  3. Laura Strockbine
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  4. Timothy A. Sato
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  5. Ky N. Clifford
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  6. Brian M. Macduff
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  7. Dirk M. Anderson
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  8. Steven D. Gimpel
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  9. Terri Davis-Smith
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  10. Charles R. Maliszewski
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  11. Edward A. Clark
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  13. Kenneth H. Grabstein
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  14. David Cosman
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  15. Melanie K. Spriggs
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Armitage, R., Fanslow, W., Strockbine, L. et al. Molecular and biological characterization of a murine ligand for CD40. Nature 357, 80–82 (1992). https://doi.org/10.1038/357080a0

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  • DOI: https://doi.org/10.1038/357080a0

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