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Structure of domain 1 of rat T lymphocyte CD2 antigen

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Abstract

THE CD2 antigen is largely restricted to cells of the T-lymphocyte lineage and has been established as an important adhesion molecule in interactions between human T lymphocytes and accessory cells1. In the adhesion reaction, CD2 on T cells binds to LFA-3 on other cells, with binding through domain 1 of CD2 (ref. 2). CD2 can also be a target for the delivery of mitogenic signals to T lymphocytes cultured with combinations of anti-CD2 antibodies3,4. Two predictions that are contradictory have been made for the structure of CD2 domain 1. One suggests an immunoglobulin (Ig) fold, on the basis of sequence patterns conserved in the Ig-superfamily (IgSF)5, whilst the other proposes a pattern of alternating α-helices and β-strands, on the basis of secondary structure predictions6. Thus CD2 domain 1 is an important test case for the validity of IgSF assignments based on sequence patterns. We report here the expression of domain 1 of rat CD2 in an Escherichia coli expression system and have determined a low-resolution solution structure by NMR spectroscopy.

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Authors and Affiliations

  1. Department of Biochemistry, University of Oxford, Oxford, 0X1 3QU, UK

    Paul C. Driscoll & Iain D. Campbell

  2. MRC Cellular Immunology Research Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford, 0X1 3RE, UK

    Jason G. Cyster & Alan F. Williams

Authors
  1. Paul C. Driscoll
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  2. Jason G. Cyster
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  3. Iain D. Campbell
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  4. Alan F. Williams
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Driscoll, P., Cyster, J., Campbell, I. et al. Structure of domain 1 of rat T lymphocyte CD2 antigen. Nature 353, 762–765 (1991). https://doi.org/10.1038/353762a0

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