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Peptide-binding specificity of the molecular chaperone BiP

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Abstract

Members of the heat-shock protein family (hsp70s) can distinguish folded from unfolded proteins. This property is crucial to the role of hsp70s as molecular chaperones and is attributable to the amino-acid specificity of the peptide-binding site. The specificity for peptide ligands is investigated using a set of peptides of random sequence but defined chain length. The peptide-binding site selects for aliphatic residues and accommodates them in an environment energetically equivalent to the interior of a folded protein.

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References

  1. Lippincott-Schwartz, J., Bonifacino, J. S., Yuan, L. C. & Klausner, R. D. Cell 54, 209–220 (1988).

    Article  CAS  PubMed  Google Scholar 

  2. Goldberg, A. L. & St John, A. C. A. Rev. Biochem. 45, 747–803 (1976).

    Article  CAS  Google Scholar 

  3. Ostermann, J., Horwich, A. L., Neupert, W. & Hartl, F. U. Nature 341, 125–30 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Bole, D. B., Hendershot, L. M. & Kearney, J. F. J. Cell biol. 102, 1558–1566 (1986).

    Article  CAS  PubMed  Google Scholar 

  5. Rothman, J. E. Cell 59, 591–601 (1989).

    Article  CAS  PubMed  Google Scholar 

  6. Gething, M.-J., McCammon, K. & Sambrook, J. Cell 46, 939–950 (1986).

    Article  CAS  PubMed  Google Scholar 

  7. Chirico, W. J., Waters, M. G. & Blobel, G. Nature 332, 800–805 (1988).

    Article  ADS  Google Scholar 

  8. Deshaies, R. J., Koch, B. D., Werner-Washburne, M., Craig, E. A. & Schekman, R. Nature 332, 800–804 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Kang, P.-J. et al. Nature 348, 137–143 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Beckmann, R. P., Mizzen, L. A. & Welch, W. J. Science 248, 850–853 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Palleros, D. R., Welch, W. J. & Fink, A. L. Proc. natn. Acad. Sci. U.S.A. 88, 5719–5723 (1991).

    Article  ADS  CAS  Google Scholar 

  12. Flynn, G. C., Chappell, T. G. & Rothman, J. E. Science 245, 385–390 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Nozaki, Y. & Tanford, C. J. biol. Chem. 246, 2211–2217 (1971).

    CAS  PubMed  Google Scholar 

  14. Matsumaura, M., Becktel, W. J. & Matthews, B. J. Nature 334, 406–410 (1988).

    Article  ADS  Google Scholar 

  15. Miller, S., Janin, J., Lesk, A. M. & Chothia, C. J. molec. Biol. 196, 641–656 (1987).

    Article  CAS  PubMed  Google Scholar 

  16. Lim, W. A. & Sauer, R. T. Nature 339, 31–36 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Elsenberg, D., Weiss, R. M. & Terwilliger, T. C. Proc. natn. Acad. Sci. U.S.A. 81, 140–144 (1984).

    Article  ADS  Google Scholar 

  18. Kapper, M. H. Biochem. biophys. Res. Commun. 78, 1018–1024 (1977).

    Article  Google Scholar 

  19. Lewis, M. J. & Pelham, H. R. B. EMBO J. 4, 3137–3143 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Pelham, H. R. B. Cell 46, 959–961 (1986).

    Article  CAS  PubMed  Google Scholar 

  21. Chappell, T. G., Konforti, B. B., Schmid, S. L. & Rothman, J. E. J. biol. Chem. 262, 746–751 (1987).

    CAS  PubMed  Google Scholar 

  22. Flajnik, M. F., Canel, C. C., Kramer, J. & Kasahara, M. Proc. natn. Acad. Sci. U.S.A. 88, 537–541 (1991).

    Article  ADS  CAS  Google Scholar 

  23. Flajnik, M. F., Canel, C., Kramer, J. & Kasahara, M. Immunogenetics (in the press).

  24. Rotzschke, O. et al. Nature 348, 252–254 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Schumacher, T. N. M. et al. Nature 350, 703–706 (1991).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Falk, K., Rotzschke, O., Stevanovic, S., Jung, G. & Rammensee, H.-G. Nature 351, 290–296 (1991).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Devlin, J. J., Panganiban, L. C. & Devlin, P. E. Science 249, 404–406 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Scott, J. K. & Smith, G. P. Science 249, 386–390 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Fodor, S. P. A. et al. Science 251, 767–773 (1991).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Chang, C. D. & Meienhofer, J. Int. J. Pept. Protein Res. 11, 246–249 (1975).

    Article  Google Scholar 

  31. Cresfield, A. M., Stein, W. H. & Moore, S. J. biol. Chem. 238, 618–621 (1963).

    Google Scholar 

  32. Tack, B. F., Dean, J., Eilat, D., Lorenz, P. & Schechter, A. N. J. biol. Chem 255, 8842–8847 (1980).

    CAS  PubMed  Google Scholar 

  33. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. biol. Chem. 256, 7990–7997 (1981).

    CAS  PubMed  Google Scholar 

  34. Pohl, J., Pereira, H. A., Martin, N. M. & Spitznagel, J. K. FEBS Lett. 272, 200–204 (1990).

    Article  CAS  PubMed  Google Scholar 

  35. Tanford, C. J. Am. Chem. Soc. 84, 4240–4247 (1962).

    Article  CAS  Google Scholar 

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Author notes

  1. Jan Pohl: Microchemical Facility, Emory University School of Medicine, Atlanta, Georgia 30322, USA

  2. Mark T. Flocco: Microchemistry Core Facility, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA

Authors and Affiliations

  1. Program in Cellular Biochemistry and Biophysics, Rockefeller Research Laboratory, Sloan-Kettering Institute, 1275 York Avenue, New York, New York, 10021, USA

    Gregory C. Flynn, Jan Pohl, Mark T. Flocco & James E. Rothman

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  1. Gregory C. Flynn
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  2. Jan Pohl
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  3. Mark T. Flocco
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  4. James E. Rothman
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Flynn, G., Pohl, J., Flocco, M. et al. Peptide-binding specificity of the molecular chaperone BiP. Nature 353, 726–730 (1991). https://doi.org/10.1038/353726a0

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  • DOI: https://doi.org/10.1038/353726a0

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