Abstract
The engrailed gene product of Drosophila specifies the fate of a subset of cells in each segment. Our studies of engrailed regulation suggest that fate determination is an elaborate, multistep process. At the time in embryogenesis when the engrailed-dependent cell fate is probably determined, four modes of control act in an overlapping progression to govern engrailed expression. After activation by pair-rule genes, both an extracellular signal, wingless, and autoregulation are required for engrailed expression. Autoregulation graduates to wingless independence, but is transient, and is superseded by an engrailed- independent mode of maintenance.
Similar content being viewed by others
References
Slack, J. M. W. From Egg to Embryo: Determinative Events in Early Development. (Cambridge University Press, Cambridge, 1983).
Garcia-Bellido, A. CIBA Fdn Symp. 29, 161–182 (1975).
Lawrence, P. A. & Morata, G. Devl Biol. 50, 321–337 (1976).
Kornberg, T., Siden, I., O'Farrell, P. & Simon, M. Cell 40, 45–53 (1985).
Garcia-Bellido, A., Ripoll, P. & Morata, G. Nature 245, 251–253 (1973).
Garcia-Bellido, A., Ripoll, P. & Morata, G. Devl Biol. 48, 132–147 (1976).
Kornberg, T. Proc. natn. Acad. Sci. U.S.A. 78, 1095–1099 (1981).
Baker, N. E. EMBO J. 6, 1765–1773 (1987).
van den Heuvel, M., Nusse, R., Johnston, P. & Lawrence, P. A. Cell 59, 739–749 (1989).
Rijsewijk, F. et al. Cell 50, 649–657 (1987).
DiNardo S., Sher, E., Heemskerk-Jongens, J., Kassis, J. A. & O'Farrell, P. H. Nature 332, 45–53 (1988).
Martinez-Arias, A., Baker, N. & Ingham, P. W. Development 103, 157–170 (1988).
Baker, N. E. Devl Biol. 125, 96–108 (1988).
DiNardo, S., Kuner, J. M., Theis, J. & O'Farrell, P. H. Cell 43, 59–69 (1985).
Jaynes, J. B. & O'Farrell, P. H. EMBO J. 10, 1427–1433 (1991).
Poole, S. J. & Kornberg, T. B. Development suppl. 104, 85–93 (1988).
Kassis, J. A., Genes Dev. 4, 433–443 (1990).
DiNardo, S. & O'Farrell, P. H. Genes Dev. 1, 1212–1225 (1987).
Howard, K. & Ingham, P. Cell 44, 949–957 (1986).
Cline, T. Genetics 107, 231–277 (1984).
Kuziora, M. A. & McGinnis, W. Cell 55, 477–485 (1988).
Thayer, M. J. et al. Cell 58, 241–248 (1989).
Wedeen, C., Harding, K. & Levine, M. Cell 44, 739–748 (1986).
Struhl, G. & Akam, M. EMBO J. 4, 3259–3264 (1985).
Mazo, A. M., Huang, D.-K., Mozer, B. A. & Dawid, I. B. Proc. natn. Acad. Sci. U.S.A. 87, 2112–2116 (1990).
Busturia, A. & Morata, G. Development 104, 713–720 (1988).
Dura, J. M. & Ingham, P. Development 103, 733–741 (1988).
Smouse, D., Goodman, C., Mahowald, A. & Perrimon, N. Genes Dev. 2, 830–842 (1988).
Spradling, A. C. & Rubin, G. M. Science 218, 341–347 (1982).
Tautz, D. & Pfeifle, C. Chromosoma 98, 81–85 (1989).
Baker, N. E. Development 103, 289–298 (1988).
Poole, S. J., Kauvar, L. M., Drees, B. & Kornberg, T. Cell 40, 37–43 (1985).
Pirotta, V. in Vectors, A Survey of Molecular Cloning Vectors and their Uses, (eds Rodriguez, R. L. & Denhardt, D. T.) 437–456 (Butterworths. Boston, 1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Heemskerk, J., DiNardo, S., Kostriken, R. et al. Multiple modes of engrailed regulation in the progression towards cell fate determination. Nature 352, 404–410 (1991). https://doi.org/10.1038/352404a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/352404a0
- Springer Nature Limited
This article is cited by
-
Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis
Scientific Reports (2017)
-
En1 and Wnt signaling in midbrain dopaminergic neuronal development
Neural Development (2011)
-
Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish
BMC Developmental Biology (2007)
-
Feedback control of intercellular signalling in development
Nature (2000)
-
The segment polarity network is a robust developmental module
Nature (2000)