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The B-cell surface protein CD72/Lyb-2 is the ligand for CDS

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Abstract

THE glycoprotein CD5 is expressed on the surface membrane of all mature T cells1 and a small proportion of B lymphocytes1,2. Its exact role in immune interactions is still unknown. Studies3–10indicate that CD5 functions both in mice and humans as a receptor, delivering co-stimulatory signals to T cells in a manner similar to CD2 (ref. 11) and CD28 (ref. 12). Anti-CDS antibodies stimulate both T-cell proliferation mediated by CD3 in association with the T-cell receptor and secretion of interleukin-2 and expression of its receptor, as well as inducing an increase in intracellular Ca2+concentration (refs 5–10). To identify the ligand for CD5 we purified the human CD5 protein, labelled it with biotin and used it as a probe. Here we report that CD5 specifically interacts with the cell-surface protein CD72 exclusive to B cells. This interaction is blocked by anti-CD72 antibodies, but not by any other anti-B-cell antibodies. Moreover, non-B cells (mouse L-cell fibroblasts and human Jurkat T cells) expressing a transfected human CD72 complementary DNA could bind to the CD5–biotin conjugate. The results demonstrate that the B-cell surface protein CD72 (Lyb-2 in mice) is the ligand for CD5.

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References

  1. Wang, C. Y., Good, R. A., Ammizate, P., Dymbert, G. & Evans, R. J. exp. Med. 151, 1539–1544 (1980).

    Article  CAS  Google Scholar 

  2. Hayakawa, K. & Hardy, R. R. A. Rev. Immun. 6, 197–218 (1988).

    Article  CAS  Google Scholar 

  3. Jones, N. H. et al. Nature 323, 346–349 (1986).

    Article  ADS  CAS  Google Scholar 

  4. Huang, H. S., Jones, N. H., Strominger, J. L. & Herzenberg, L. A. Proc. natn. Acad. Sci. U.S.A. 84, 204–208 (1987).

    Article  ADS  CAS  Google Scholar 

  5. Ceuppens, J. L. & Baroja, M. L. J. Immun. 137, 1816–1821 (1986).

    CAS  PubMed  Google Scholar 

  6. Ledbetter, J. A., June, C. H., Grosmaire, L. S. & Rabinovitch, P. S. Proc. natn. Acad. Sci. U.S.A. 84, 1384–1388 (1987).

    Article  ADS  CAS  Google Scholar 

  7. Nishimura, Y. et al. Eur. J. Immun. 18, 747–753 (1988).

    Article  CAS  Google Scholar 

  8. Verwilghen, J., Vandesande, R., Vandenberghe, P. & Ceuppens, J. L. Cell. Immun. 131, 109–119 (1990).

    Article  CAS  Google Scholar 

  9. Spertini, F., Stohl, W., Ramesh, N. Moody, C. & Geha, R. S. J. Immun. 146, 47–52 (1991).

    CAS  PubMed  Google Scholar 

  10. Vandenberghe, P. & Ceupppens, J. L. Eur. J. Immun. 21, 251–259 (1991).

    Article  CAS  Google Scholar 

  11. Springer, T. A. Nature 346, 425–434 (1990).

    Article  ADS  CAS  Google Scholar 

  12. Linsley, P. S., Clark, E. A. & Ledbetter, J. A. Proc. natn. Acad. Sci. U.S.A. 87, 5031–5035 (1990).

    Article  ADS  CAS  Google Scholar 

  13. Kürzinger, K. & Springer, T. A. J. biol. Chem. 257, 12412–12418 (1982).

    PubMed  Google Scholar 

  14. Martin, S. D. & Springer, T. A. Cell 51, 813–819 (1987).

    Article  Google Scholar 

  15. Taki, S., Shimamura, T., Abe, M., Shirai, T. & Takahora, Y. J. immunol. Meth. 122, 33–41 (1989).

    Article  CAS  Google Scholar 

  16. McMichael, A. J. & Gotch, F. M. in Leucocyte Typing III (ed. McMichael, A. J.) 31–62 (Oxford University Press, Oxford, 1987).

    Google Scholar 

  17. Dörken, B., Möller, P., Pezzutto, A., Schwartz-Albiez, R. & Moldenhauer, G. in Leucocyte Typing IV (eds Knapp, W. et al.) 15–32 (Oxford University Press, Oxford, 1989).

    Google Scholar 

  18. Von Hoegen, I., Nakayama, E. & Parnes, J. R. J. Immun. 144, 4870–4877 (1990).

    CAS  PubMed  Google Scholar 

  19. Von Hoegen, I., Hsieh, C., Schwarting, R. Francke, U. & Parnes, J. R. Eur. J. Immun. (in the press).

  20. Schwarting, R. & Stein, H. in Leucocyte Typing IV (eds Knapp, W. et al.) 100–102 (Oxford University Press, Oxford, 1989).

    Google Scholar 

  21. Dörken, B., Möller, P., Pezzutto, A., Schwartz-Albiez, R. & Moldenhauer, G. in Leucocyte Typing IV (eds Knapp, W. et al) 99–100 (Oxford University Press, Oxford, 1989).

    Google Scholar 

  22. Nakayama, E., von Hoegen, I. & Parnes, J. R. Proc. natn. Acad. Sci. U.S.A. 86, 1352–1356 (1989).

    Article  ADS  CAS  Google Scholar 

  23. Pezzutto, A., Dörken, B., Valentine, M. A., Shu, G. L. & Clark, E. A. in Leucocyte Typing IV (eds Knapp, W. et al.) 178–182 (Oxford University Press, Oxford, 1989).

    Google Scholar 

  24. Kamal, M., Katira, A. & Gordon, J. Eur. J. Immun. (in the press).

  25. Subbarao, B. & Mosier, D. E. J. Immun. 140, 2033–2037 (1983).

    Google Scholar 

  26. Subbarao, B. & Melchers, F. Curr. Topics microbiol. Immun. 113, 72–76 (1984).

    CAS  Google Scholar 

  27. Snow, E. C., Mond, J. J. & Subbarao, B. J. Immun. 137, 1793–1796 (1986).

    CAS  PubMed  Google Scholar 

  28. Subbarao, B., Morris, J. & Baluyut, A. R. Cell. Immun. 112, 329–342 (1988).

    Article  CAS  Google Scholar 

  29. Yakura, H., Shen, F.-W., Kaemer, M. & Boyse, E. A. J. exp. med. 153, 129–135 (1981).

    Article  CAS  Google Scholar 

  30. Subbarao, B. & Mosier, D. E. Immunol. Rev. 69, 81–97 (1982).

    Article  CAS  Google Scholar 

  31. Gunning, P., Leavitt, J., Muscat, G., Ng, S.-Y. & Kedes, L. Proc. natn. Acad. Sci. U.S.A. 84, 4831–4837 (1987).

    Article  ADS  CAS  Google Scholar 

  32. Hardin, J. A. et al. Cell Immun. 126, 304–321 (1990).

    Article  CAS  Google Scholar 

  33. Kawamura, N. et al. J. clin. Invest. 78, 1331–1338 (1986).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Haematology-lmmunology, Medical School of the Vrije Universiteit Brussel, B-1090, Brussels, Belgium

    Hilde Van de Velde, Wei Luo & Kris Thielemans

  2. Division of Immunology and Rheumatology, Stanford University Medical Center, Stanford, California, 94305, USA

    Ilka von Hoegen & Jane R. Parnes

Authors
  1. Hilde Van de Velde
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  2. Ilka von Hoegen
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  3. Wei Luo
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  4. Jane R. Parnes
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  5. Kris Thielemans
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de Velde, H., von Hoegen, I., Luo, W. et al. The B-cell surface protein CD72/Lyb-2 is the ligand for CDS. Nature 351, 662–665 (1991). https://doi.org/10.1038/351662a0

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