Abstract
IN higher plant cells, cytokinesis is achieved by new cross-wall formation mediated by the phragmoplast1, a double ring of microtubules of opposite polarity, in which the short microtubules are arranged perpendicular to the equatorial plane of the phragmoplast with their plus ends interdigitating at the plane1,2. The phragmoplast and its enclosed cell plate move out centrifugally until the mother cell divides. We report here results of a newly developed method using glycerinated cultured tobacco cells, which show that the equatorial region of the phragmoplast can translocate microtubules towards their minus ends concomitantly with tubulin polymerization at their plus ends. The translocation is induced effectively by GTP and less effectively by ATP, and is inhibited by the unhydrolysable nucleotide analogues GMP-PNP and AMP-PNP. Thus, the equatorial region of the phragmoplast seems to be associated with a mechanochemical enzyme that generates the force for microtubule translocation by hydrolysing GTP.
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References
Gunning, B. E. S. in The Cytoskeleton in Plant Growth and Development (ed. Lloyd, C. W.) 229–292 (Academic, London, 1982).
Euteneuer, U., Jackson, W. T. & McIntosh, J. R. J. Cell Biol. 94, 644–653 (1982).
Hoffman-Berling, H. Biochim. biophys. Acta 14, 182–194 (1954).
Masuda, H. & Cande, W. Z. Cell 49, 193–202 (1987).
Mitchison, T. J. & Kirschner, M. W. J. Cell Biol. 101, 766–777 (1985).
Shpetner, H. S. & Vallee, R. B. Cell 59, 421–432 (1989).
Obar, R. A., Collins, C. A., Hammarback, J. A., Shpetner, H. S. & Vallee, R. B. Nature 347, 256–261 (1990).
Kakimoto, T. & Shibaoka, H. Protoplasma (Suppl. 2) 95–103 (1988).
Weingarten, M. D., Lockwood, A. H., Hwo, S. Y. & Kirschner, M. W. Proc. natn. Acad. Sci. U.S.A. 722, 1858–1862 (1975).
Keith, C. H., Feramisco, J. R. & Shelanski, M. J. J. Cell Biol. 88, 234–240 (1981).
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Asada, T., Sonobe, S. & Shibaoka, H. Microtubule translocation in the cytokinetic apparatus of cultured tobacco cells. Nature 350, 238–241 (1991). https://doi.org/10.1038/350238a0
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DOI: https://doi.org/10.1038/350238a0
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