Abstract
HEAT-shock proteins have been implicated in assembly of protein complexes1, correct protein folding2 and uptake of proteins into organdies3–4. In Escherichia coli, the heat-shock protein DnaJ and the HspTO homologue, DnaK, act together to disassemble a protein complex involved in bacteriophage λ replication5. We report the identification of SCJ1, a gene in the yeast Saccharomyces cerevisiae that encodes a homologue of the bacterial DnaJ protein. SCJ1 was identified by a genetic screen in which increased expression of candidate genes results in missorting of a nuclear-targeted test protein. The predicted amino-acid sequence of SCJ1 is 37% identical to the entire E. coli DnaJ protein. Hybridization experiments indicate that there is a family of yeast genes related to SCJ1. These findings suggest that the HspTO DnaK-DnaJ interaction is general to eukaryotes.
Similar content being viewed by others
References
Ellis, R. J. & Hemmingsen, S. M. Trends biochem. Sci. 14, 339–342 (1989).
Rothman, J. E. Cell 59, 591–601 (1989).
Deshaies, R. J., Koch, B. D., Werner-Washburne, M., Craig, E. A. & Schekman, R. Nature 332, 800–805 (1988).
Chirico, W. J., Waters, M. G. & Blobel, G. Nature 332, 805–810 (1988).
Liberek, K., Georgopoulos, C. & Zylicz, M. Proc. natn. Acad. Sci. U.S.A. 85, 6632–6636 (1988).
Sadler, I. et al. J. Cell Biol. 109, 2665–2675 (1989).
Ohki, M., Tamura, F., Nishimura, S. & Uchida, H. J. biol. Chem. 261, 1782–1785 (1986).
Bardwell, J. C. A. et al. J. biol. Chem. 261, 1782–1785 (1986).
Lathigra, R. B., Young, D. B., Sweetser, D. & Young, R. A. Nucleic Acids Res. 16, 1636–1638 (1988).
Rothblatt, J. A., Deshaies, R. J., Sanders, S. L., Daum, G. & Schekman, R. J. Cell Biol. 109, 2641–2652 (1989).
Hartl, F.-U., Pfanner, N., Nicholson, D. W. & Neupert, W. Biochim. biophys. Acta 989, 1–45 (1989).
von Heijne, G. Curr. top. Membranes Transp. 24, 151–179 (1985).
Munro, S. & Pelham, H. R. B. Cell 46, 291–300 (1986).
Lebowitz, J. H., Zylicz, M., Georgopoulos, C. & McMacken, R. Proc. natn. Acad. Sci. U.S.A. 82, 3988–3992 (1985).
Zylicz, M., Ang, D., Liberek, K. & Georgopoulos, C. EMBO J. 8, 1601–1608 (1989).
Georgopoulos, C. P. & Herskowitz, I. in The Bacteriophage Lambda (ed. Hershey, A. D.) 553–564 (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1971).
Gaitanaris, G. M., Papavassiliou, A. G., Rubock, P., Silverstein, S. J. & Gottesman, M. E. Cell 61, 1013–1020 (1990).
Kang, P.-J. et al. Nature 348, 137–142 (1990).
Ito, H., Fukado, Y., Murata, K. & Kimura, A. J. Bacteriol. 153, 163–168 (1983).
Carlson, M. & Botstein, D. Cell 28, 145–154 (1982).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Devereux, J., Haeberli, P. & Smithies, O. Nucleic Acids Res. 12, 387–395 (1984).
Sherman, F., Fink, G. R. & Hicks, J. B. Methods in Yeast Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1986).
Maniatis, T., Fritsch, E. F. and Sambrook, J. Molecular Cloning: a Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1982).
Walworth, N. C., Goud, B., Ruohola, H. and Novick, P. J. Methods Cell Biol. 31, 335–354 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blumberg, H., Silver, P. A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast. Nature 349, 627–630 (1991). https://doi.org/10.1038/349627a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/349627a0
- Springer Nature Limited
This article is cited by
-
Transcriptomic analyses giving insights into molecular regulation mechanisms involved in cold tolerance by Epichloë endophyte in seed germination of Achnatherum inebrians
Plant Growth Regulation (2016)
-
Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding
Nature (1992)