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Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung

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Abstract

PLATELET-activating factor (PAF), a unique phospholipid mediator, possesses potent proinflammatory, smooth-muscle contractile and hypotensive activities, and appears to be crucial in the pathogenesis of bronchial asthma and in the lethality of endotoxin and anaphylactic shock1–3. Despite this, little is known of the molecular properties of the PAF receptor and related signal transduction systems. Although several lines of evidence suggest that activation of the PAF receptor stimulates phospholipase C and subsequent inositol trisphosphate formation through G protein(s)4,5, the PAF receptor and calcium channel are reported to show a close relation2,6. As a first approach to cloning lipid autacoid receptors, we have isolated complementary DNA for the PAF receptors. Our strategy involved gene expression in Xenopus laevis oocytes and electrophysiological detection of PAF-induced responses. Sequence analysis indicates that the receptor belongs to the superfamily of G protein-coupled receptors.

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Author notes

  1. Takao Shimizu: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Physiological Chemistry and Nutrition, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan

    Zen-ichiro Honda, Motonao Nakamura, Ichiro Miki, Michiko Minami, Tsuyoshi Watanabe, Yousuke Seyama & Takao Shimizu

  2. Department of Neurobiology, Institute of Brain Research, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan

    Haruo Okado

  3. Department of Internal Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan

    Kohji Ito & Terumasa Miyamoto

  4. Protein Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka, 565, Japan

    Hiroyuki Toh

  5. On leave from Department of Internal Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan

    Zen-ichiro Honda

Authors
  1. Zen-ichiro Honda
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  2. Motonao Nakamura
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  3. Ichiro Miki
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  4. Michiko Minami
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  5. Tsuyoshi Watanabe
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  6. Yousuke Seyama
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  7. Haruo Okado
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  8. Hiroyuki Toh
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  9. Kohji Ito
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  10. Terumasa Miyamoto
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  11. Takao Shimizu
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Honda, Zi., Nakamura, M., Miki, I. et al. Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung. Nature 349, 342–346 (1991). https://doi.org/10.1038/349342a0

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  • DOI: https://doi.org/10.1038/349342a0

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