The RNA polymerases from T7 and related bacteriophages, in conjunction with elements of DNA and RNA viruses, can be used in novel ways for expression of genes in mammalian cells.
References
Fuerst, T.R., Niles, E.G., Studier, F.W. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 83, 8122–8126 (1986).
Mizukami, T., Fuerst, T.R., Berger, E.A. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 85, 9273–9277 (1988).
Whitt, M.A., Chong, L. & Rose, J.K. J. Virol. 63, 3569–3578 (1989).
Pattnaik, A.K. & Wertz, G.W. J. Virol. 64, 2948–2957 (1990).
Fuerst, T.R., Earl, P.L. & Moss, B. Molec. cell. Biol. 7, 2538–2544 (1987).
Barrett, N. et al. AIDS Res. Human Retroviruses 5, 159–171 (1989).
Fuerst, T.R. & Moss, B. J. molec. Biol. 206, 333–348 (1989).
Elroy-Stein, O., Fuerst, T.R. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 86, 6126–6130 (1989).
Rich, D.P. et al. Nature 347, 358–363 (1990).
Fuerst, T.R., Fernandez, M.P. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 86, 2549–2553 (1989).
Alexander, W.A., Moss, B. & Fuerst, T.R. (in preparation).
Elroy-Stein, O. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 87, 6743–6747 (1990).
Deuschle, U. et al. Proc. natn. Acad. Sci. U.S.A. 86, 5400–5404 (1989).
Zhou, Y., Giordano, T.J., Durbin, R.K. & McAllister, W.T. Molec. cell. Biol. 10, 4529–4537 (1990).
Lieber, A., Kiessling, U. & Strauss, M. Nucleic Acids Res. 17, 8485–8493 (1989).
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Moss, B., Elroy-Stein, O., Mizukami, T. et al. New mammalian expression vectors. Nature 348, 91–92 (1990). https://doi.org/10.1038/348091a0
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DOI: https://doi.org/10.1038/348091a0
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