Use of Escherichia coli as an expression host has opened up new possibilities in antibody research and its applications. It greatly facilitates rational engineering and random mutagenesis.
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Plückthun, A. Antibodies from Escherichia coli. Nature 347, 497–498 (1990). https://doi.org/10.1038/347497a0
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DOI: https://doi.org/10.1038/347497a0
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