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Identification of an actin-binding protein from Dictyostelium as elongation factor 1a

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Abstract

INDIRECT evidence has implicated an interaction between the cytoskeleton and the protein synthetic machinery1–8. Two recent reports have linked the elongation factor la (EF-la) which is involved in protein synthesis, with the microtubular cytoskeleton7–8.In situ hybridization has, however, revealed that the messages for certain cytoskeletal proteins are preferentially associated with actin filaments6. ABP-50 is an abundant actin filament bundling protein of native relative molecular mass 50,000 (50K)9 isolated from Dictyostelium discoideum. Immunofluorescence studies show that ABP-50 is present in filopodia and other cortical regions that contain actin filament bundles9,10. In addition, ABP-50 binds to monomeric actin in the cytosol of unstimulated cells and the association of ABP-50 with the actin cytoskeleton is regulated during chemotaxis10. Through complementary DNA sequencing and subsequent functional analysis, we have identified ABP-50 as D. discoideum EF-la. The ability of EF-la to bind reversibly to the actin cytoskeleton upon stimulation could provide a mechanism for spatially and temporally regulated protein synthesis in eukaryotic cells.

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Authors and Affiliations

  1. Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA

    Fan Yang, Mark Demma, Vivien Warren, Suranganie Dharmawardhane & John Condeelis

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  1. Fan Yang
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  2. Mark Demma
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  3. Vivien Warren
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  4. Suranganie Dharmawardhane
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  5. John Condeelis
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Yang, F., Demma, M., Warren, V. et al. Identification of an actin-binding protein from Dictyostelium as elongation factor 1a. Nature 347, 494–496 (1990). https://doi.org/10.1038/347494a0

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  • DOI: https://doi.org/10.1038/347494a0

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