Abstract
THE specificity of vesicular transport in a cell is determined by the formation of vesicles with specific contents from a donor compartment and their selective fusion with the appropriate acceptor compartment. Several of the latter fusion steps have been investigated in detail using cell-free systems1–9, and work with these systems as well as genetic evidence has revealed a role for GTP-binding proteins in membrane fusion processes7–15. We have reconstituted the formation of constitutive secretory vesicles and immature secretory granules from the trans Golgi network in a cell-free system16. We show here that the budding of both types of post-Golgi vesicles is inhibited by non-hydrolysable analogues of GTP, which suggests a more widespread role for GTP-binding proteins in membrane traffic than previously assumed.
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Tooze, S., Weiss, U. & Huttner, W. Requirement for GTP hydrolysis in the formation of secretory vesicles. Nature 347, 207–208 (1990). https://doi.org/10.1038/347207a0
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DOI: https://doi.org/10.1038/347207a0
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