Skip to main content

Advertisement

SpringerLink
Log in

No evidence for expression of the Insulin-regulatable glucose transporter in endothelial cells

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

A MAJOR effect of insulin is to increase glucose transport in muscle and fat1,2. A family of genes encoding distinct mammalian glucose transporters has recently been elucidated3–9. One of these, the insulin-regulatable glucose transporter (IRGT)3,10, is primarily expressed in muscle and fat, tissues that exhibit insulin-dependent glucose transport. Insulin promotes glucose transport in these tissues by stimulating movement of the glucose transporter from an intracellular location to the plasma membrane3,10–14. Recent studies15, however, suggest that an additional effect of insulin in these tissues may be the facilitation of glucose transport, presumably across capillary endothelium. This hypothesis is based on the localization of the IRGT in endothelial cells specific to muscle and adipose tissue. We report here, however, on morphological and biochemical studies using several different IRGT-specific antibodies in which we could not reproduce these results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Morgan, H. E. & Whitfield, C. F. Curr. Top. Membranes Transp. 4, 255–303 (1973).

    Article  CAS  Google Scholar 

  2. Elbrink, J. & Bihler, I. Science 188, 1177–1184 (1975).

    Article  ADS  CAS  Google Scholar 

  3. James, D. E., Strube, M. & Mueckler, M. Nature 338, 83–87 (1989).

    Article  ADS  CAS  Google Scholar 

  4. Birnbaum, M. J. Cell 57, 305–315 (1989).

    Article  CAS  Google Scholar 

  5. Charron, M. J., Brosius, F. C., Alper, S. L. & Lodish, H. F. Proc. natn. Acad. Sci. U.S.A. 86, 2535–2539 (1989).

    Article  ADS  CAS  Google Scholar 

  6. Kaestner, K. H. et al. Proc. natn. Acad. Sci. U.S.A. 86, 3150–3154 (1989).

    Article  ADS  CAS  Google Scholar 

  7. Fukumoto, H. et al. J. biol. Chem. 264, 7776–7779 (1989).

    CAS  Google Scholar 

  8. Mueckler, M. et al. Science 229, 941–945 (1985).

    Article  ADS  CAS  Google Scholar 

  9. Thorens, B., Sarkar, H. K., Kaback, H. R. & Lodish, H. F. Cell 55, 281–290 (1988).

    Article  CAS  Google Scholar 

  10. James, D. E., Brown, R., Navarro, J. & Pilch, P. F. Nature 333, 183–185 (1988).

    Article  ADS  CAS  Google Scholar 

  11. Suzuki, K. & Kono, T. Proc. natn. Acad. Sci. U.S.A. 77, 2542–2545 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Cushman, S. W. & Wardzala, L. J. J. biol. Chem. 255, 2758–2762 (1980).

    Google Scholar 

  13. Wardzala, L. J. & Jeanrenaud, B. J. biol. Chem. 256, 7090–7093 (1981).

    CAS  PubMed  Google Scholar 

  14. Watanabe, T., Smith, M. M., Robinson, F. W. & Kono, T. J. biol. Chem. 259, 13117–13133 (1984).

    CAS  PubMed  Google Scholar 

  15. Vilaro, S., Palacin, M., Pilch, P. F., Testar, X. & Zorzano, A. Nature 342, 789–800 (1989).

    Article  ADS  Google Scholar 

  16. Maruyama, I., Bell, C. E. & Majerus, P. W. J. Cell Biol. 101, 363–371 (1985).

    Article  CAS  Google Scholar 

  17. Cheung, J. Y., Conover, C., Regen, D. M., Whitfield, C. F. & Morgan, H. E. Am. J. Physiol. 234, E70–78 (1978).

    CAS  PubMed  Google Scholar 

  18. Kuikka, J., Levin, M. & Bassingthwaighte, J. B. Am. J. Physiol. 250, H29–42 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Slot, J. W., Geuze, H. J. & Weerkamp, A. H. J. meth. Microbiol. 20, 211–236 (1988).

    Article  Google Scholar 

  20. Kern, P. A., Knedler, A. & Eckel, R. H. J. clin. Invest. 71, 1822–1829 (1983).

    Article  CAS  Google Scholar 

  21. Kumeda, T., Dittman, W. A. & Majerus, P. W. Blood 71, 728–733 (1987).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, Medical School, University of Utrecht, 3584 CX, Utrecht, The Netherlands

    Jan W. Slot & Hans J. Geuze

  2. Department of Neurology, University of Rochester, Rochester, New York, 14642, USA

    Richard Moxley

  3. Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri, 63110, USA

    David E. James

Authors
  1. Jan W. Slot
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard Moxley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hans J. Geuze
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David E. James
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Slot, J., Moxley, R., Geuze, H. et al. No evidence for expression of the Insulin-regulatable glucose transporter in endothelial cells. Nature 346, 369–371 (1990). https://doi.org/10.1038/346369a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/346369a0

  • Springer Nature Limited

This article is cited by

Search

Navigation