Abstract
THE replication of retroviruses and pararetroviruses (such as caulimo- and hepadnaviruses)1 involves the production of terminally redundant genomic-length RNA (Fig. 1). The sequence repeated at both ends of the RNA (the R region) contains a polyadenylation signal, and for production of full-length RNA the version of this at the 5′ end of the template must be bypassed by RNA polymerase, but the version at the 3′ end must be recognized2–5. This implies that the position of the polyadenylation signal determines its efficiency, and we report here experiments aimed at investigating the basis of this phenomenon. Our results with cauliflower mosaic virus6 suggest that proximity to the transcription initiation site inhibits messenger RNA 3′-end processing directed by polyadenylation signals.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Temin, H. M. Nature 339, 254–255 (1989).
Stoltzfus, C. M. Adv. Virus Res. 35, 1–38 (1988).
Dougherty, J. P. & Temin, H. M. Proc. natn. Acad. Sci. U.S.A. 84, 1197–1201 (1987).
Benz, E. W. Jr. Wydro, R. M., Nadal-Ginard, B. & Dina, D. Nature 288, 665–669 (1980).
Seiki, M., Hattori, S., Hirayama, Y. & Yoshida, M. Proc. natn. Acad. Sci. U.S.A. 80, 3618–3622 (1983).
Bonneville, J. M., Hohn, T. & Pfeiffer, P. RNA Genetics Vol. 2 (eds Domingo, E., Holland, J. J. & Ahlquist, P.) 23–42 (CRC Press, Baton Rouge, 1988).
Bevan, M., Barnes, W. & Chilton, M. D. Nucleic Acids Res. 11, 369–385 (1982).
Depicker, A., Stachel, S., Dhaese, P., Zambryski, P. & Goodman, H. M. J. molec. appl. Genet. 1, 561–574 (1982).
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Guilley, H., Dudley, R. K., Jonard, G., Balazs, E. & Richards, K. E. Cell 30, 763–773 (1982).
Whitelaw, E. & Proudfoot, N. EMBO J. 5, 2915–2922 (1986).
Logan, J., Falck-Pedersen, E., Darnell, J. E. Jr & Shenk, T. Proc. natn. Acad. Sci. U.S.A. 84, 8306–8310 (1987).
Connelly, S. & Manley, J. L. Genes Dev. 2, 440–452 (1988).
Lanoix, J. & Acheson, N. H. EMBO J. 7, 2515–2522 (1988).
Bunick, D., Zandomeni, R., Ackerman, S. & Weinmann, R. Cell 29, 877–886 (1982).
Coppola, J. A., Field, A. S. & Luse, D. S. Proc. natn. Acad. Sci. U.S.A. 80, 1251–1255 (1983).
Denome, R. M. & Cole, C. N. Molec. Cell Biol. 8, 4829–4839 (1988).
Pietrzak, M., Shillito, R. D., Hohn, T. & Potrykus, I. Nucleic Acids Res. 14, 5857–5868 (1986).
Jefferson, R. A., Kavanagh, T. A. & Bevan, M. W. EMBO J. 6, 3901–3907 (1987).
Marsh, J. L., Erfle, M. & Wykes, E. J. Gene 32, 481–485 (1984).
Franck, G., Guilley, M., Jonard, G., Richards, K. & Hirth, L. Cell 21, 285–294 (1980).
Vankan, P., Edoh, D. & Filipowicz, W. Nucleic Acids Res. 16, 10425–10440 (1988).
Goodall, G. J. & Filipowicz, W. Cell 58, 473–483 (1989).
Howarth, A. J., Gardner, J. C., Messing, J. & Shepherd, R. J. Virology 112, 678–685 (1981).
Fromm, M., Taylor, L. P. & Walbot, V. Proc. natn. Acad. Sci. U.S.A. 82, 5824–5828 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sanfaçon, H., Hohn, T. Proximity to the promoter inhibits recognition of cauliflower mosaic virus polyadenylation signal. Nature 346, 81–84 (1990). https://doi.org/10.1038/346081a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/346081a0
- Springer Nature Limited
This article is cited by
-
Synthetic memory circuits for stable cell reprogramming in plants
Nature Biotechnology (2022)
-
Plant mRNA 3?-end formation
Plant Molecular Biology (1996)
-
Analysis of 3′ terminals of human immunodeficiency virus type 1 transcripts in persistently infected cells
Virus Genes (1993)
-
Transcriptional and post-transcriptional regulation of gene expression in plants
Plant Molecular Biology (1992)