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Chloroplast DNA sequence from a Miocene Magnolia species

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Abstract

DNA has been successfully extracted from several samples of preserved tissue, the oldest so far reported originating from a 13,000-year-old ground sloth1.But severe damage to the preserved DNA, primarily due to oxidation of the pyrimidines1, has prevented the acquisition of sequence data from ancient samples except in a few cases2–4. We report here the extraction of DNA from fossil leaf samples from the Miocene Clarkia deposit (17-20 My r old), the amplification of an 820-base pair (bp) DNA fragment from the chloroplast gene rbcL from a fossil of the genus Magnolia, and its subsequent sequencing. The sequence was verified by comparison with published and unpublished rbcL sequences. These results extend our ability to analyse ancient DNA and may open new avenues into problems in palaeobotany, biogeography, and in the calibration of mutation rates.

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Authors and Affiliations

  1. Department of Botany and Plant Sciences, University of California, Riverside, California, 92521, USA

    Edward M. Golenberg, Michael T. Clegg, Mary Durbin & David Henderson

  2. Department of Botany, University of Georgia, Athens, Georgia, 30602, USA

    David E. Giannasi

  3. Department of Geology and Geological Engineering, University of Idaho, Moscow, Idaho, 83843, USA

    Charles J. Smiley

  4. DNAX Research Institute, Palo Alto, California, 94304-1104, USA

    Gerard Zurawski

Authors
  1. Edward M. Golenberg
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  2. David E. Giannasi
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  3. Michael T. Clegg
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  4. Charles J. Smiley
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  5. Mary Durbin
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  6. David Henderson
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  7. Gerard Zurawski
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Golenberg, E., Giannasi, D., Clegg, M. et al. Chloroplast DNA sequence from a Miocene Magnolia species. Nature 344, 656–658 (1990). https://doi.org/10.1038/344656a0

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