Abstract
THE antigen receptors on mature B lymphocytes are membrane-bound immunoglobulins of the IgM and IgD classes1,2 whose cross-linking by polyvalent antigens results in B-cell proliferation and differentiation3. How these membrane-bound immunoglobulin chains, which lack a cytoplasmic tail, generate a cell activation signal is not at present known. We now show that the IgM molecule is non-covalently associated in the membrane of B cells with two proteins of relative molecular mass 34,000 (Mr 34 K; IgM-α) and 39 K (Ig-β) which form a disulphide-linked heterodimer. Surface expression of IgM seems to require the formation of an appropriate complex between IgM and the heterodimer. A transfection experiment indicates that IgM-α is the product of mb-l, a B-cell-specific gene4encoding a transmembrane protein with sequence homology5 to proteins of the T-cell antigen receptor–CD3 complex6–8.
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Goding, J. W., Scott, D. W. & Layton, J. E. Immunol. Rev. 37, 152–186 (1977).
Vitetta, E. S. & Uhr, J. E. Eur. J. Immun. 6, 140–143 (1976).
Cambier, J. C. & Ransom, J. T. A. Rev. Immun. 5, 175–199 (1987).
Sakaguchi, N., Kashiwamura, S., Kimoto, M., Thalmann, P. & Melchers, F. EMBO J. 7, 3457–3464 (1988).
Reth, M. Nature 338, 383 (1989).
Clevers, H., Alarcon, B., Wileman, T. & Terhorst, C. A. Rev. Immun. 6, 629–662 (1988).
Samelson, L. E., Harford, J. B. & Klausner, R. D. Cell 43, 223–231 (1985).
Weiss, A. et al. A. Rev. Immun. 4, 593–619 (1986).
Sitia, R., Neuberger, M. & Milstein, C. EMBO J. 6, 3969–3977 (1988).
Hombach, J., Sablitzky, F., Rajewsky, K. & Reth, M. J. exp. Med. 167, 652–657 (1988).
Hombach, J., Leclercq, L., Radbruch, A., Rajewsky, K. & Reth, M. EMBO J. 7, 3451–3456 (1988).
Kim, K. J., Kanellopoulos-Langevin, C., Mervin, R. M., Sachs, D. H. & Asofsky, R. J. Immun. 122, 549–554 (1979).
Picard, D. & Schaffner, W. Proc. natn. Acad. Sci. U.S.A. 80, 417–421 (1983).
Reth, M., Petrac, E., Wiese, P., Lobel, L. & Alt, F. EMBO J. 6, 3299–3305 (1987).
Arnold, B., Burgert, H. G., Archibald, A. C. & Kvist, S. Nucleic Acids Res. 12, 9473–9486 (1984).
Blank, U. et al. Nature 337, 187–189 (1989).
Sussman, J. J. et al. Cell 52, 85–95 (1988).
Wienands, J., Hombach, J. & Reth, M. EMBO J. (in the press).
Harnett, M. & Klaus, G. G. B. J. Immun. 140, 3135–3139 (1988).
Southern, P. J. & Berg, P. J. molec. appl. Genet. 1, 327–342 (1982).
Sefton, B. M., Wickups, G. G. & Burge, B. W. J. Virol. 11, 730–735 (1973).
Blum, H., Beier, H. & Gross, H. J. Electrophoresis 8, 93–99 (1987).
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Hombach, J., Tsubata, T., Leclercq, L. et al. Molecular components of the B-cell antigen receptor complex of the IgM class. Nature 343, 760–762 (1990). https://doi.org/10.1038/343760a0
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DOI: https://doi.org/10.1038/343760a0
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