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Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist

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Abstract

Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-lp. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.

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Author notes

  1. Charles H. Hannum

    Present address: , DNAX 901 California Avenue, Palo Alto, California, 94304-1104, USA

  2. William P. Arend: Division of Rheumatology, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA

Authors and Affiliations

  1. Synergen Inc., 1885 33rd Street, Boulder, Colorado, 80301, USA

    Stephen P. Eisenberg, Ron J. Evans, William P. Arend, Evie Verderber, Michael T Brewer, Charles H. Hannum & Robert C. Thompson

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  1. Stephen P. Eisenberg
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  2. Ron J. Evans
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  3. William P. Arend
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  4. Evie Verderber
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  5. Michael T Brewer
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  6. Charles H. Hannum
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  7. Robert C. Thompson
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Eisenberg, S., Evans, R., Arend, W. et al. Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist. Nature 343, 341–346 (1990). https://doi.org/10.1038/343341a0

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