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Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein

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Abstract

THE process of nuclear pre-messenger RNA splicing is similar in Saccharomyces cerevisiae and metazoan cells in that the two-step mechanism is identical and the reaction occurs in a large ribonucleoprotein complex, the spliceosome1. Little is known, however, about the degree of conservation of splicing factors other than of the small nuclear RNAs (snRNAs). Yeast counterparts of the metazoan spliceosomal snRNAs (Ul, U2, U4, U5 and U6) have been identified2 but, with the exception of U6, the yeast snRNAs are larger and sequence similarity is limited to short regions. By using antibodies against the yeast PRP8 protein, a pre-mRNA splicing factor of relative molecular mass 280,000 (Mr280K) stably associated with U5 small nuclear ribonucleoproteins (snRNPs)3,4, we have now identified an immunologically related protein in HeLa cell nuclear extracts. The HeLa cell protein has an Mr greater than 200K and is associated with purified 20S U5 snRNPs. This is the first report of phylogenetic conservation between yeast and man of a protein splicing factor.

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Author notes

  1. Gordon J. Anderson

    Present address: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, 0X1 3QU, UK

Authors and Affiliations

  1. Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK

    Gordon J. Anderson & Jean D. Beggs

  2. Institut für Molekularbiologie und Tumorforschung der Philipps-Universität Marburg, Emil-Mannkopff-Strasse 2, D-3550, Marburg, FRG

    Montserrat Bach & Reinhard Lührmann

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  1. Gordon J. Anderson
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  2. Montserrat Bach
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  3. Reinhard Lührmann
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  4. Jean D. Beggs
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Anderson, G., Bach, M., Lührmann, R. et al. Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein. Nature 342, 819–821 (1989). https://doi.org/10.1038/342819a0

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