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Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor

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Abstract

The sequence of 5,037 amino acids composing the ryanodine receptor from rabbit skeletal muscle sarcoplasmic reticulum has been deduced by cloning and sequencing the complementary DNA. The predicted structure suggests that the calcium release channel activity resides in the C-terminal region of the receptor molecule, whereas the remaining portion constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum and the transverse tubule.

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References

  1. Endo, M. Physiol. Rev. 57, 71–108 (1977).

    Article  CAS  PubMed  Google Scholar 

  2. Martonosi, A. N. Physiol. Rev. 64, 1240–1320 (1984).

    Article  CAS  PubMed  Google Scholar 

  3. Franzini-Armstrong, C. J. Cell Biol. 47, 488–499 (1970).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Inui, M., Saito, A. & Fleischer, S. J. biol. Chem. 262, 1740–1747 (1987).

    CAS  PubMed  Google Scholar 

  5. Imagawa, T., Smith, J. S., Coronado, R. & Campbell, K. P. J. biol. Chem. 262, 16636–16643 (1987).

    CAS  PubMed  Google Scholar 

  6. Lai, F. A., Eriokson, H. P., Rousseau, E., Liu, Q-Y. & Meissner, G. Nature 331, 315–319 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Saito, A., Inui, M., Radermacher, M., Frank, J. & Fleischer, S. J. Cell Biol. 107, 211–219 (1988).

    Article  CAS  PubMed  Google Scholar 

  8. Hymel, L., Inui, M., Fleisher, S. & Schindler, H. Proc. natn. Acad. Sci. U.S.A. 85, 441–445 (1988).

    Article  ADS  CAS  Google Scholar 

  9. Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).

    Article  CAS  PubMed  Google Scholar 

  10. Breathnach, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).

    Article  CAS  Google Scholar 

  11. Kadonaga, J. T., Jones, K. A. & Tjian, R. Trends biochem. Sci. 11, 20–23 (1986).

  12. Korczak, B. et al. J. biol. Chem. 263, 4813–4819 (1988).

    CAS  PubMed  Google Scholar 

  13. Ellis, S. B. et al. Science 241, 1661–1664 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Wagenknecht, T. et al. Nature 338, 167–170 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  PubMed  Google Scholar 

  18. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978).

    Article  CAS  Google Scholar 

  19. Noda, M. et al. Nature 312, 121–127 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Meissner, G. Biochemistry 25, 244–251 (1986).

    Article  CAS  PubMed  Google Scholar 

  21. Kretsinger, R. H. A. Rev. Biochem. 45, 239–266 (1976).

    Article  CAS  Google Scholar 

  22. Wierenga, R. K. & Hol, W. G. J. Nature 302, 842–844 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Blumenthal, D. K. et al. Proc. natn. Acad. Sci. U.S.A. 82, 3187–3191 (1985).

    Article  ADS  CAS  Google Scholar 

  24. Numa, S. Chemica Scripta. 26B, 173–178 (1986).

  25. Grenningloh, G. et al. Nature 328, 215–220 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Schofield, P. R. et al. Nature 328, 221–227 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Tanabe, T. et al. Nature 328, 313–318 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Tempel, B. L. Papazian, D. M., Schwarz, T. L., Jan, Y. N. & Jan, L. Y. Science 237, 770–775 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Paul, D. L. J. Cell Biol. 103, 123–134 (1986).

    Article  CAS  PubMed  Google Scholar 

  30. Smith, J. S. et al. J. gen. Physiol. 92, 1–26 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Brisson, A. & Unwin, P. N. T. Nature 315, 474–477 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  32. Imoto, K. et al. Nature 335, 645–648 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  33. Hille, B. Ionic Channels of Excitable Membranes (Sinauer, Sunderland, Massachusetts, 1984).

    Google Scholar 

  34. Tanabe, T., Beam, K. G., Powell, J. A. & Numa, S. Nature 336, 134–139 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  35. Rios, E. & Brum, G. Nature 325, 717–720 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  36. Block, B. A., Imagawa, T., Campbell, K. P. & Franzini-Armstrong, C. J. Cell Biol. 107, 2587–2600 (1988).

    Article  CAS  PubMed  Google Scholar 

  37. Berridge, M. J. A. Rev. Biochem. 56, 159–193 (1987).

    Article  CAS  Google Scholar 

  38. Supattapone, S., Worley, P. F., Baraban, J. M. & Snyder, S. H. J. biol. Chem. 263, 1530–1534 (1988).

    CAS  PubMed  Google Scholar 

  39. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  40. Saito, A., Seiler, S., Chu, A. & Fleischer, S. J. Cell Biol. 99, 875–885 (1984).

    Article  CAS  PubMed  Google Scholar 

  41. Costello, B., Chadwick, C., Saito, A., Chu, A., Maurer, A. & Fleischer, S. J. Cell Biol. 103, 741–753 (1986).

    Article  CAS  PubMed  Google Scholar 

  42. Kawakami, K. et al. Nature 316, 733–736 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  43. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  PubMed  Google Scholar 

  44. Maniatis, T. et al. Cell 15, 687–701 (1978).

    Article  CAS  PubMed  Google Scholar 

  45. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  46. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  PubMed  Google Scholar 

  47. Fukuda, K. et al. Nature 335, 355–358 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  48. Köhler, G. & Milstein, C. Nature 256, 495–497 (1975).

    Article  ADS  PubMed  Google Scholar 

  49. Towbin, H., Staehelin, T. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4354 (1979).

    Article  ADS  CAS  Google Scholar 

  50. Noda, M. et al. Nature 305, 818–823 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  51. Toh, H., Hayashida, H. & Miyata, T. Nature 305, 827–829 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Departments of Medical Chemistry and Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto, 606, Japan

    Hiroshi Takeshima, Seiichiro Nishimura, Takeshi Matsumoto, Hiroyuki Ishida & Shosaku Numa

  2. Department of Pathology, Institute for Virus Research, Kyoto University, Kyoto, 606, Japan

    Masamichi Ueda & Masao Hanaoka

  3. Department of Biochemistry, Miyazaki Medical College, Miyazaki, 889-16, Japan

    Kenji Kangawa, Naoto Minamino & Hisayuki Matsuo

  4. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose

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  1. Hiroshi Takeshima
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  2. Seiichiro Nishimura
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  3. Takeshi Matsumoto
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  4. Hiroyuki Ishida
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  11. Shosaku Numa
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Takeshima, H., Nishimura, S., Matsumoto, T. et al. Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor. Nature 339, 439–445 (1989). https://doi.org/10.1038/339439a0

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  • DOI: https://doi.org/10.1038/339439a0

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