Skip to main content
SpringerLink
Log in

Avoiding false positives with PCR

  • Product Review
  • Published:

From Nature

View current issue Submit your manuscript

The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Erlich, H.A., Gelfand, D.H. & Saiki, R.K. Nature 331, 461–462 (1988).

    Article  ADS  Google Scholar 

  2. Saiki, R.K. et al. Science 239, 487–491 (1988).

    Article  ADS  CAS  Google Scholar 

  3. Faloona, F. & Mullis, K. Meth. Enzym. 155, 335–350 (1987).

    Article  Google Scholar 

  4. Li, H. et al. Nature 335, 414–417 (1988).

    Article  ADS  CAS  Google Scholar 

  5. Lo, Y.-M.D., Mehal, W.Z. & Fleming, K.A. Lancet 2, 679 (1988).

    Article  CAS  Google Scholar 

  6. Ou, C. et al. Science 239, 295–297 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Higuchi, R., von Beriodingen, C.H., Sensabaugh, G.F. & Erlich, H.A. Nature 332, 543–546 (1988).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Infectious Diseases and Human Genetics, Cetus Corporation, Emery-ville, California, 94608, USA

    S. Kwok & R. Higuchi

Authors
  1. S. Kwok
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Higuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kwok, S., Higuchi, R. Avoiding false positives with PCR. Nature 339, 237–238 (1989). https://doi.org/10.1038/339237a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/339237a0

  • Springer Nature Limited

This article is cited by

Search

Navigation