Abstract
THE initiation of a specific immune response is believed to require not only activation through antigen-specific receptors on T cells and B cells but also antigen-independent interactions between accessory molecules1. One such molecule is LFA-1, which enhances the avidity of interactions between T cells and antigen-presenting cells2–4, and is possibly involved in signal transduction across the T-cell membrane5. Intercellular adhesion molecule-1 (ICAM-1), a surface glycoprotein of relative molecular mass (Mr) 80,000–110,000, has been defined as a ligand for LFA-16,7, and has been shown to participate in the interaction between T cells and monocytes8. The determination of the precise contribution of such accessory molecules to antigen presentation, however, is complicated by the need to analyse against a background of multiple molecular interactions. We have investigated the role of LFA-1/ICAM-1 interactions in antigen presentation directly by quantifying the contribution of ICAM-1 expression to T-cell stimulation using L-cell transfectants that co-express ICAM-1 and HLA-DR. In the case of transfectants expressing modest levels of HLA-DR, co-expression of ICAM-1 is critical for effective HLA class H-restricted and allospecific T-cell activation, pointing to an important role for ICAM-1 in the induction of T-cell responses.
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Springer, T. A., Dustin, M. L., Kishimoto, T. K. & Marlin, S. D. A. Rev. Immun. 5, 223–252 (1987).
Gougeon, M.-L., Bismuth, G. & Theze, J. cell. Immun. 95, 75–83 (1985).
Dougherty, G. J. & Hogg, N. Eur. J. Immun. 17, 943–947 (1987).
Shaw, S. et al. Nature 323, 262–264 (1986).
van Noesel, C. et al. Nature 333, 850–852 (1988).
Marlin, S. & Springer, T. A. Cell 51, 813–819 (1987).
Simmons, D., Makgoba, M. W. & Seed, B. Nature 331, 624–627 (1988).
Dougherty, G. J., Murdoch, S. & Hogg, N. Eur. J. Immun. 18, 35–39 (1988).
Wilkinson, D. et al. J. exp. Med. 167, 1442–1458 (1988).
Young, J. A. T., Wilkinson, D., Bodmer, W. F. & Trowsdale, J. Proc. natn. Acad. Sci., U.S.A. 84, 4929–4933 (1987).
Rogelj, S., Weinberg, R. A., Fanning, P. & Klagsburn, M. Nature 331, 173–175 (1988).
Rothlein, R., Dustin, M. L., Marlin, S. D. & Springer, T. A. J. Immun. 137, 1270–1274 (1986).
Golde, W. T. et al. J. exp. Med. 161, 635–640 (1985).
Barbosa, J. A. et al. Proc. natn. Acad. Sci., U.S.A. 81, 7549–7553 (1984).
Eckels, D. D., Sell, T. W., Long, E. O. & Sekaly, R. P. Hum. Immun. 21, 173–181 (1988).
Hildreth, J. E. K., Gotch, F. M., Hildreth, P. D. K. & McMichael, A. J. Eur. J. Immun. 13, 202–208 (1983).
Lampson, L. A. & Levy, R. J. Immun. 125, 293–299 (1980).
Marrack, P. & Kappler, J. Nature 332, 840–843 (1988).
Matis, L. A., Soerger, S. B., McElliot, D. L., Fink, P. J. & Hedrick, S. M. Cell 51, 59–69 (1988).
Collins, T. et al. Proc. natn. Acad. Sci., U.S.A. 81, 4917–4921 (1984).
Dustin, M. L., Rothlein, R., Bhan, A. K., Dinarello, C. A. & Springer, T. A. J. Immun. 137, 245–255 (1986).
Radka, S. F., Charron, D. J. & Brodsky, F. M. Hum. Immun. 16, 390–400 (1986).
Dustin, M. L., Singer, K. H., Tuck, D. T. & Springer, T. A. J. exp. Med. 167, 1323–1340 (1988).
Bottazzo, G. F., Pujol-Borrell, R., Hanafusa, T. & Feldmann, M. Lancet ii, 1115–1117 (1983).
Gregory, C. D., Murray, R. J., Edwards, C. F. & Rickinson, A. B. J. exp. Med. 167, 1811–1824 (1988).
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Altmann, D., Hogg, N., Trowsdale, J. et al. Cotransfection of ICAM-1 and HLA-DR reconstitutes human antigen-presenting cell function in mouse L cells. Nature 338, 512–514 (1989). https://doi.org/10.1038/338512a0
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DOI: https://doi.org/10.1038/338512a0
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