Abstract
Current candidate vaccines fail to protect primates against challenge with human immunodeficiency virus (HIV) in the presence of antibody responses1; this underlines the importance of studying cell-mediated immunity to HIV and identifying specific epitopes that stimulate cytotoxic T lymphocytes (CTL). Using a recombinant vaccinia virus to express the gag protein of HIV-1 we found HLA class-I-restricted gag-specific CTL in thirteen out of fifteen healthy HIV seropositive patients. We then used short synthetic peptides in the lysis assay2–5 to screen for gag CTL epitopes. In one patient we have identified a peptide in p24 that is recognized by CTL in association with HLA-B27. This peptide, and further peptide sequences defined by these methods, could be incorporated in vaccines designed to induce cell-mediated immunity against HIV.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berman, P. W. et al. Proc. natn. Acad. Sci. U.S.A. 85, 5200–5204 (1988).
Townsend, A. R. M. et al. Cell 44, 959–968 (1986).
McMichael, A. J., Gotch, F. M. & Rothbard, J. J. exp. Med. 164, 1397–1406 (1987).
Gotch, F. M., Rothbard, J., Howland, K., Townsend, A. & McMichael, A. Nature 326, 881–882 (1987).
Braciale, T. J. et al. J. exp. Med. 166, 678–692 (1987).
Walker, B. D. et al. Nature 328, 345–348 (1987).
Walker, B. D. et al. Science 240, 64–66 (1988).
Plata, F. et al. Nature 328, 348–351 (1987).
Takahashi, H. et al. Proc. natn. Acad. Sci. U.S.A. 85, 3105–3109 (1988).
Weber, J. et al. Lancet i, 119–122 (1987).
Hahn, B. H. et al. Nature 312, 166–169 (1984).
Chakrabarti, S. Brechling, K. & Moss, B. Molec. Cell. Biol. 5, 3403–3409 (1985).
Smith, G. L., Murphy, B. R. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 80, 7155–7159 (1983).
Sanchez-Pescador, R. et al. Science 227, 484–492 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nixon, D., Townsend, A., Elvin, J. et al. HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides. Nature 336, 484–487 (1988). https://doi.org/10.1038/336484a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/336484a0
- Springer Nature Limited
This article is cited by
-
Host genetic variation and HIV disease: from mapping to mechanism
Immunogenetics (2017)
-
Disease progression despite protective HLA expression in an HIV-infected transmission pair
Retrovirology (2015)
-
Development of prophylactic vaccines against HIV-1
Retrovirology (2013)
-
The impact of differential antiviral immunity in children and adults
Nature Reviews Immunology (2012)
-
Modification of a loop sequence between α-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vifand CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells
Retrovirology (2009)