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NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A

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Abstract

The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional 1H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure.

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University School of Medicine, Stanford, California, 94305, USA

    Jayant B. Udgaonkar & Robert L. Baldwin

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  1. Jayant B. Udgaonkar
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  2. Robert L. Baldwin
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Udgaonkar, J., Baldwin, R. NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A. Nature 335, 694–699 (1988). https://doi.org/10.1038/335694a0

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