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Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells

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Abstract

The regulation of the subclass of immunoglobulin secreted by B cells has been studied in vitro in polyclonal systems using mitogens, such as lipopolysaccharide (LPS), to bypass the requirement for cognate interaction between antigen-specific T and B cells. In these systems, interleukin-(IL)-4 induces the secretion of IgG1, (ref. 1) and IgE (ref. 2); IL-5 enhances the secretion of IgA3,4, and interferon-γ (IFN-γ) enhances the secretion of IgG2a (ref. 5). Clones of murine TH cells can be divided into two subsets, TH1 and TH2 (ref. 6). Both subsets synthesize IL-3 and granulocyte-monocyte colony-stimulating factor (GM-CSF), but only TH1 clones produce IL-2, IFN-γ, and lymphotoxin (LT) and TH2 clones produce IL-4 and IL-5 (ref. 7). We have examined the role of clones of antigen-specific TH1 and TH2 cells in the regulation of the subclasses of IgG antibody secreted by antigen-specific B cells. Our results show that both types of TH cells induce the secretion of IgM and IgG3, whereas clones of TH1 and TH2 cells specifically induce antigen-specific B cells to secrete IgG2a and IgG1, respectively. We also demonstrate that regulation of commitment to the secretion of a particular IgG isotype occurs in two distinct stages: cognate interaction between T and B cells and interaction between T-cell-derived lymphokines and B cells.

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Authors and Affiliations

  1. Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75235, USA

    Virginia M. Sanders, Rafael Fernandez-Botran & Ellen S. Vitetta

  2. Immunology Graduate Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75235, USA

    Tracy L. Stevens, Alexis Bossie & Ellen S. Vitetta

  3. DNAX Research Institute, Palo Alto, California, 94304, USA

    Robert L. Coffman & Timothy R. Mosmann

Authors
  1. Tracy L. Stevens
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  2. Alexis Bossie
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  3. Virginia M. Sanders
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  4. Rafael Fernandez-Botran
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  5. Robert L. Coffman
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  6. Timothy R. Mosmann
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  7. Ellen S. Vitetta
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Stevens, T., Bossie, A., Sanders, V. et al. Regulation of antibody isotype secretion by subsets of antigen-specific helper T cells. Nature 334, 255–258 (1988). https://doi.org/10.1038/334255a0

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  • DOI: https://doi.org/10.1038/334255a0

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