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Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product

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Abstract

One of the cellular targets implicated in the process of transformation by the adenovirus E1A proteins is a 105K cellular protein. Previously, this protein had been shown to form stable protein/protein complexes with the E1A polypeptides but its identity was unknown. Here, we demonstrate that it is the product of the retinoblastoma gene. The interaction between E1A and the retinoblastoma gene product is the first demonstration of a physical link between an oncogene and an anti-oncogene.

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References

  1. Courtneidge, S. A. & Smith, A. E. Nature 303, 435–439 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Lane, D. P. & Crawford, L. V. Nature 278, 261–263 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Linzer, D. I. H. & Levine, A. J. Cell 17, 43–52 (1979).

    Article  CAS  PubMed  Google Scholar 

  4. McCormick, F. & Harlow, E. J. Virol. 34, 213–224 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Sarnow, P., Ho, Y. S., Williams, J. & Levine, A. J. Cell 28, 387–394 (1982).

    Article  CAS  PubMed  Google Scholar 

  6. Bolen, J. B. et al. Cell 38, 767–777 (1984).

    Article  CAS  PubMed  Google Scholar 

  7. Courtneidge, S. EMBO J. 4, 1471–1477 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Oren, M., Maltzman, W. & Levine, A. J. Molec. cell. Biol. 1, 101–110 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Reich, N. C., Oren, M. & Levine, A. J. Molec. cell. Biol. 3, 2143–2150 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Houweling, A., van den Elsen, P. J. & van der Eb, A. J. Virology 105, 537–550 (1980).

    Article  CAS  PubMed  Google Scholar 

  11. Ruley, E. Nature 304, 602–606 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Cone, R., Grodzicker, T. & Jaramillo, M. Molec. cell. Biol. 8, 1036–1044 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. van den Elsen, P. J., Houweling, A. & van der Eb, A. J. Virology 128, 377–390 (1983).

    Article  CAS  PubMed  Google Scholar 

  14. Berk, A. A. Rev. Genet. 20, 45–79 (1986).

    Article  CAS  Google Scholar 

  15. Yee, S. & Branton, P. E. Virology 147, 142–153 (1985).

    Article  CAS  PubMed  Google Scholar 

  16. Harlow, E., Whyte, P., Franza, B. R. & Schley, C. Molec. cell. Biol. 6, 1579–1589 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Whyte, P., Ruley, E. & Harlow, E. J. Virol. 62, 257–265 (1988).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Klein G. Science 238, 1539–1545 (1988).

    Article  ADS  Google Scholar 

  19. Hansen, M. F. & Cavenee, W. K. Cell 53, 172–173 (1988).

    Article  CAS  Google Scholar 

  20. Vogel, F. Hum. Genet. 52, 1–54 (1979).

    Article  CAS  PubMed  Google Scholar 

  21. Cavenee, W. K. et al. Nature 305, 779–783 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Murphee, A. L. & Benedict, W. F. Science 223, 1028–1033 (1984).

    Article  ADS  Google Scholar 

  23. Dryja, T. P., Rapaport, J. M., Joyce, J. M. & Petersen, R. A. Proc. natn. Acad. Sci. U.S.A. 83, 7391–7394 (1986).

    Article  ADS  CAS  Google Scholar 

  24. Abramson, D. H., Ellsworth, R. M., Kitchin, D. & Tung, G. Ophthalmology 91, 1351–1355 (1984).

    Article  CAS  PubMed  Google Scholar 

  25. Friend, S. H. et al. Nature 323, 643–646 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Fung, Y. et al. Science 236, 1657–1661 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Lee, W. et al. Science 235, 1394–1399 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  28. Friend, S. H. et al. Proc. natn. Acad. Sci. U.S.A. 84, 9059–9063 (1987).

    Article  ADS  CAS  Google Scholar 

  29. Lee, W. et al. Nature 329, 642–645 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Harlow, E., Franza, B. R. & Schley, C. J. Virol. 55, 533–546 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  31. Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).

    Article  CAS  PubMed  Google Scholar 

  32. Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. W. J. Virol. 39, 861–869 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Berk, A. J. & Sharp, P. A. Cell 14, 695–711 (1978).

    Article  CAS  PubMed  Google Scholar 

  34. Chow, L. T., Broker, T. R. & Lewis, J. B. J. molec. Biol. 134, 265–303 (1979).

    Article  CAS  PubMed  Google Scholar 

  35. Kitchingman, G. R. & Wesphal, H. J. molec. Biol. 137, 23–48 (1980).

    Article  CAS  PubMed  Google Scholar 

  36. Stephens, C. & Harlow, E. EMBO J. 6, 2027–2035 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Ulfendahl, P. J. et al. EMBO J. 6, 2037–2052 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Yee, S., Rowe, D. T., Remblay, M. L., McDermot, M. & Branton, P. E. J. Virol. 46, 1003–1013 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  39. Spindler, K. R., Rosser, D. S. E. & Berk, A. J. J. Virol. 49, 132–141 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Tsukamoto, A. S., Ponicelli, A., Berk, A. J. & Gaynor, R. B. J. Virol. 59, 14–22 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  41. Stephens, C., Franza, B. R., Schley, C. & Harlow, E. in Cancer Cells 4, DNA Tumor Viruses (eds Botchan, M., Grodzicker, T. & Sharp, P. A.) 429–434 (Cold Spring Harbor, New York, 1986).

    Google Scholar 

  42. Tremblay, M. L., McGlade, C. J., Gerber, G. E. & Branton, P. E. J. biol. Chem. 263, 6375–6383 (1988).

    CAS  PubMed  Google Scholar 

  43. Krieg, P. A. & Melton, D. A. Nucleic Acids Res. 12, 7057–7070 (1984).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Braithwaite, A. W. et al. J. Virol. 45, 192–199 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  45. Stabel, S., Argos, P. & Philipson, L. EMBO J. 4, 2329–2336 (1985).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Kaczmarek, L., Ferguson, B., Rosenberg, M. & Baserga, R. Virology 152, 1–10 (1986).

    Article  CAS  PubMed  Google Scholar 

  47. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  PubMed  Google Scholar 

  48. Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U. K. J. biol. Chem. 252, 1102–1106 (1977).

    CAS  PubMed  Google Scholar 

  49. Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W. & Howard, J. C. Nature 266, 550–552 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  50. Liu, F., Zinnecker, M., Hamaoka, T. & Katz, D. H. Biochemistry 18, 690–697 (1979).

    Article  CAS  PubMed  Google Scholar 

  51. Lischwe, M. A. & Ochs, D. Analyt. Biochem. 127, 453–457 (1982).

    Article  CAS  PubMed  Google Scholar 

  52. Burnette, W. N. Analyt. Biochem. 112, 195–203 (1981).

    Article  CAS  PubMed  Google Scholar 

  53. Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).

    Article  CAS  PubMed  Google Scholar 

  54. Borrelli, E., Hen, R. & Chambon, P. Nature 312, 608–612 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  55. Velcich, A. & Ziff, E. Cell 40, 705–716 (1985).

    Article  CAS  PubMed  Google Scholar 

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Author information

Author notes

  1. Peter Whyte

    Present address: Fred Hutchinson Cancer Center, 1124 Columbia Street, Seattle, Washington, 98104, USA

  2. Margaret Raybuck

    Present address: Amersham International pic., Forest Farm Industrial Estate, Whitchurch, Cardiff, UK

  3. Ed Harlow: To whom correspondence should be addressed.

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA

    Peter Whyte, Karen J. Buchkovich, Margaret Raybuck & Ed Harlow

  2. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, 02142, USA

    Jonathan M. Horowitz, Stephen H. Friend & Robert A. Weinberg

  3. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Jonathan M. Horowitz, Stephen H. Friend & Robert A. Weinberg

  4. Division of Hematology-Oncology, The Children's Hospital, Dana-Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Stephen H. Friend

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  1. Peter Whyte
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  2. Karen J. Buchkovich
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  3. Jonathan M. Horowitz
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  4. Stephen H. Friend
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  5. Margaret Raybuck
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  6. Robert A. Weinberg
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  7. Ed Harlow
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Whyte, P., Buchkovich, K., Horowitz, J. et al. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature 334, 124–129 (1988). https://doi.org/10.1038/334124a0

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  • DOI: https://doi.org/10.1038/334124a0

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