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Real-time imaging of evoked activity in local circuits of the salamander olfactory bulb

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Abstract

The encoding of olfactory information in the central nervous system (CNS) depends on spatially distributed patterns of activity generated simultaneously in many neuronal circuits11–4. Optical neurophysiological recording permits analysis of neural activity non-invasively and with high spatial and temporal resolution5. Here, a video method for imaging voltage-sensitive dye fluorescence in vivo is used to map neuronal activity in local circuits of the salamander olfactory bulb. The method permits the imaging of simultaneous ensemble transmembrane activity in real time. After electrical stimulation of the olfactory nerve, activity spreads centripetally from the sites of synaptic input to generate non-homogeneous response patterns that are presumably mediated by local circuits within the bulbar layers. The results also show the overlapping temporal sequences of activation of cell groups in each layer. The method thus provides high resolution, sequential video images of the spatial and temporal progression of transmembrane events in neuronal circuits after afferent stimulation and offers the opportunity for studying ensemble events in other brain regions.

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References

  1. Kauer, J. S. in Neurobiology of Taste and Smell (eds Finger, T. E. & Silver, W. L.) 205–231 (Wiley, New York, 1987).

    Google Scholar 

  2. Shepherd, G. M. in Taste, Olfaction and the Central Nervous System (ed. Pfaff, D. W.) 307–332 (Rockefeller, New York, 1985).

    Google Scholar 

  3. Gesteland, R. C., Lettvin, J. Y. & Pitts, W. H. J. Physiol., Lond. 181, 525–559 (1965).

    Article  CAS  Google Scholar 

  4. Doving, K. B. Acta physiol. scand. 130, 285–298 (1987).

    Article  CAS  Google Scholar 

  5. Cohen, L. B., Salzberg, B. M. & Grinvald, A. A. Rev. Neurosci. 1, 171–182 (1978).

    Article  CAS  Google Scholar 

  6. Rall, W. & Shepherd, G. M. J. Neurophysiol. 31, 884–915 (1968).

    Article  CAS  Google Scholar 

  7. Shepherd, G. M. Physiol. Rev. 52, 864–917 (1972).

    Article  CAS  Google Scholar 

  8. Kauer, J. S. J. Physiol., Lond. 243, 695–715 (1974).

    Article  CAS  Google Scholar 

  9. Hamilton, K. A. & Kauer, J. S. Brain Res. 338, 181–185 (1985).

    Article  CAS  Google Scholar 

  10. Harrison, S. A. & Scott, J. W. J. Neurophysiol. 56, 1571–1589 (1986).

    Article  CAS  Google Scholar 

  11. Macrides, F. & Chorover, S. Science 175, 84–87 (1972).

    Article  ADS  CAS  Google Scholar 

  12. Meredith, M. & Moulton, D. G. J. gen. Physiol. 71, 615–643 (1978).

    Article  CAS  Google Scholar 

  13. Stewart, W. B., Kauer, J. S. & Shepherd, G. M. J. comp. Neurol. 185, 715–734 (1979).

    Article  CAS  Google Scholar 

  14. Mori, K. Prog. Neurobiol. 29, 320–375 (1987).

    Article  Google Scholar 

  15. Grinvald, A., Anglister, L., Freeman, J. A., Hildesheim, R. & Manker, A. Nature 308, 848–850 (1984).

    Article  ADS  CAS  Google Scholar 

  16. Kauer, J. S. & Shepherd, G. M. Brain Res. 85, 108–113 (1975).

    Article  CAS  Google Scholar 

  17. Getchell, T. V. & Shepherd, G. M. J. Physiol., Lond. 282, 521–540 (1978).

    Article  CAS  Google Scholar 

  18. Ohrbach, H. S. & Cohen, L. B. J. Neurosci. 3, 2251–2262 (1983).

    Article  Google Scholar 

  19. Kauer, J. S., Senseman, D. & Cohen, L. B. Brain Res. 418, 255–261 (1987).

    Article  CAS  Google Scholar 

  20. Cohen, L. B. & Salzberg, B. M. Rev. Physiol. Biochem. Pharmac. 83, 35–88 (1978).

    Article  CAS  Google Scholar 

  21. Kauer, J. S. Anat. Rec. 200, 331–336 (1981).

    Article  CAS  Google Scholar 

  22. Stewart, W. B. & Pedersen, P. E. Brain Res. 411, 248–258 (1987).

    Article  CAS  Google Scholar 

  23. Grinvald, A., Lieke, E., Frostig, R. D., Gilbert, C. D. & Wiesel, T. N. Nature 324, 361–364 (1986).

    Article  ADS  CAS  Google Scholar 

  24. Blasdel, G. G. & Salama, G. Nature 321, 579–585 (1986).

    Article  ADS  CAS  Google Scholar 

  25. Konnerth, A. & Orkand, R. Neurosci. Lett. 66, 49–54 (1986).

    Article  CAS  Google Scholar 

  26. Konnerth, A., Obaid, A. L. & Salzberg, B. M. Biol. Bull. 169, 553 (1985).

    Google Scholar 

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  1. Departments of Neurosurgery,and Anatomy and Cell Biology, New England Medical Center and Tufts Medical School, Boston, Massachusetts, 02111, USA

    John S. Kauer

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  1. John S. Kauer
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Kauer, J. Real-time imaging of evoked activity in local circuits of the salamander olfactory bulb. Nature 331, 166–168 (1988). https://doi.org/10.1038/331166a0

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  • DOI: https://doi.org/10.1038/331166a0

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