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Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA

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Abstract

E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells1; it is also called uvomorulin2, L-CAM (ref. 3), cell-CAM 120/80 (ref. 4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies1, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell–cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA, and were morphologically transformed so as to form colonies in which cells were tightly connected to each other.

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References

  1. 1. Yoshuia-Noro. C, Si,/uki. N. &. Takeichi, M. Devl Diol 101, l^-?7 (1984). 2. Hyal, P., Murdlo, D, Ba">ir;ei, G, & Jacob. F. Cell 21. 927-934 (1980). 3. Galim. W .!.. hdo!m;m, G M <y O.-iminpham.. B. A Pr<.". nain. Acad. Sci. U.S.A. 80, 10/S- !04J i >;>H3<, 4. Damsky. I.. H,. Kitha, J.. Seller, D., Knudsen. K. & Buck, (.. A Cell 34, 455-466 (1983). 5. hnbof. B. A... \oihcrs. H I'.. < ioodman, S. 1'.. & Bin.hrnoicr. W. Cell 35, 667-675 (1983). 6. Peyn.-r.-x N.; Umvard, O. % Jacnb, F Pro, n.-itr.. Acud. Sa. U.S.A. 82, 8067-8071 (1985). 7. Shirayoshi, Y, Nose. A., lw;"aki, K. & Takeichi. M. Cell Struct. Funct. 11, 245-252 (1986). 8. Shirajoshi, Y. ct al. FMBO J 5, 24*5-2488 (1986). 9. Kozak. M. Nuclin' Acid'- Ka.. *2. Sf>7 -H'72 H9-S4). 10. Yon Heijne, G. /. mkf. fiio!. 184, 99-10? (1985). 11 .Wickens, M. > i Mephor. P. SWUM 226, !M5-!05l iWHAt. 12. Gallin, W. J. Sofkin, B. C.. fldelnan. G. M & Cunningha^. B. A. Pntc. natn. Acad. Sci. U.S.A. 84, -SOS. 2812 (H'8/^. 13.Nose, A. A: Takvivhi, M. J. Cell B\"l. 103. 2649.-205X H986). 14.Urushihara, H., O-^ki. H. S. k Takeichi, M. Derl Bio!. 70/206-216 (1979). 15.Sander, F., Nickleii. S. A C'oulson A, R Proc. natn. Acad Sa. U.S.A. 74. 5463-5467 (1977). 16. Xie, H. Cell Struct. Funtt. 8, 315-325 U9H4). Gorman. 17. C. V\:A C.'onwg: a Pineal Approach Vol. M. (ed. Glover, D. M.) 143-190 (1RL, Oxford, 19S1), 18.Southern, P. J. <1 flcr", P. ./. m,y/".v appl (tenet. 1. 327 341 (1982). 19. Nicolas, J. F. & Bery. P Terai^carcinnma Stem Ccih (cds Silver, L. M, Martin, G. R. & Strickiand, S.) 46'-)".4s.^ ".old Sprnit, Harbor Laboratory, New York, 1983). 20.Mjrmuis, T, Fius-.h, i:,. F. <k Sarnbrnok, J. Molecular Cloning, a Laboratory Manual (Cold Spr-np Harbor [,ahor;!U'r>, N,-w Vork. 1982;.

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Nagafuchi, A., Shirayoshi, Y., Okazaki, K. et al. Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature 329, 341–343 (1987). https://doi.org/10.1038/329341a0

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