Abstract
The liver phase of development of malaria parasites has been studied only recently and remains poorly understood compared to the other stages such as sporozoïtes, merozoïtes and gametes1,2. Access to liver forms of Plasmodium falciparum has been improved by the development of in vivo3 and in vitro4 propagation methods, but the yield of mature schizonts remains limited and does not allow a detailed antigenic analysis. To date, only immunofluores-cence assays (IFA) have permitted a description of a species and liver-stage-specific antigen(s) (LSA; ref. 3). Monospecific antibodies to these antigens have not been obtained due either to difficulty in immunizing mice (against LSA), or to poor stability of human monoclonal antibodies. Therefore, as a means of characterizing the LSA, we used an alternative immunological approach to identify clones of the corresponding LSA genes. We describe here the isolation of a DNA sequence coding for a P. falciparum liver-stage-specific antigen composed of repeats of 17 amino-acids, which is immunogenic in man.
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Miller, L. H., David, P. H. & Hadley, T. J. Phil. Trans. R. Soc. B307, 99–115 (1984).
Nussenzweig, V. & Nussenzweig, R. S. Cell 42, 401–403 (1985).
Druilhe, P., Puebla, R. M., Miltgen, F. Perrin, L. & Gentilini, M. Am. J. trop. Med. Hyg. 33, 336–341 (1984).
Mazier, D. et al. Science 227, 440–442 (1985).
Rosario, V. Science 212, 137–138 (1981).
Young, R. A. & Davies, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 1194–1198 (1983).
Ozaki, L. S. et al. J. Immun. Meth. 89, 213–219 (1986).
Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321.
Smith, G. E. & Summers, M. D. Analyt. Biochem. 109, 123–129 (1980).
Schwartz, D. C. & Cantor, C. R. Cell 37, 67–65 (1984).
Carle, G. F. & Olson, M. V. Nucleic Acids Res. 12, 5647–5664 (1984).
Van der Ploeg, L. H. T. et al. Science 229, 658–661 (1985).
Kemp, D. J. et al. Nature 315, 347–350 (1985).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A 74, 5463–5674 (1977).
Cowman, A. F. et al. Molec. biol. Med. 2, 207–221 (1984).
Stahl, H. D. et al. Proc. natn. Acad. Sci. U.S.A. 82, 543–547 (1985).
Kemp, D. J. et al. Parasitology 91, S83–S1O8 (1986).
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Guerin-Marchand, C., Druilhe, P., Galey, B. et al. A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning. Nature 329, 164–167 (1987). https://doi.org/10.1038/329164a0
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DOI: https://doi.org/10.1038/329164a0
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