Abstract
Both human and murine cytotoxic T cells (CTL) elicited in response to infection with influenza A viruses have been shown to be specific for internal viral proteins, such as the matrix and nucleoprotein1–3. Individual CTL epitopes have been identified in the nucleoprotein by successfully substituting short synthetic peptides for the intact virus in the preparation of target cells in cytotoxicity assays4. The defined peptide epitopes have each been recognized by CTL in association with individual class I major histocompatibility complex (MHC) proteins, H–2Db (ref. 4), H–2Kk (ref. 5), H–2Kd (Taylor, P. et al., unpublished data) and HLA-B37 (refs 4,6). A logical strategy to investigate the molecular details of the interaction between antigen and MHC class I proteins would be to define an epitope recognized by the MHC class I molecule HLA-A2. This is because the amino-acid sequence is known, several variants of A2 have been characterized and the protein has been purified and crystallized7–11. Here we describe a peptide derived from the influenza matrix protein that is recognized by human CTL in association with the HLA-A2 molecule.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Townsend, A. R. M. & Skehel, J. J. J. exp. Med 160, 552–563 (1984).
Yewdell, J. W., Bennink, J. R., Smith, G. L. & Moss, B. Proc. natn. Acad. Sci. U.S.A. 82, 1785–1789 (1985).
Gotch, F. M., McMichael, A. J., Smith, G. L. & Moss, B. J. exp. Med. 165, 408–416 (1987).
Townsend, A. R. M. et al. Cell 44, 959–968 (1986).
Bastin, J. M., Rothbard, J., Davey, J. & Townsend, A. R. M. J. exp. Med. (in the press).
McMichael, A. J., Gotch, F. M. & Rothbard, J. J. exp. Med. 164, 1397–1406 (1986).
Lopez de Castro, J. A., Strominger, J. L., Strong, D. M. & Orr, H. Proc. natn. Acad. Sci. U.S.A. 79, 3813–3817 (1982).
Krangel, M. S., Taketani, S., Biddison, W. E., Strong, D. M. & Strominger, J. L. Biochemistry 21, 6313–6321 (1982).
Koller, B. H. & Orr, H. T. J. Immun. 134, 2727–2733 (1985).
Taketani, S. M., Krangel, M. S., Pious, D. & Strominger, J. L. J. Immun. 131, 2935–2938 (1983).
Bjorkman, P. J., Strominger, J. L. & Wiley, D. C. J. molec. Biol. 186, 205–210 (1985).
Rothbard, J. B. & Taylor, W. Nature (submitted).
Winter, G. & Fields, S. Nucleic Acids Res. 8, 1965–1974 (1980).
Gotch, F. M. et al. Immunogenetics 21, 11–23 (1985).
Babbitt, B. P., Allen, P. M., Matsueda, G., Haber, E. & Unanue, E. R. Nature 317, 359–361 (1985).
Watts, T. H., Gaub, H. E. & McConnell, H. M. Nature 320, 179–181 (1986).
Buus, S. et al. Proc. natn. Acad. Sci. U.S.A. 83, 3968–3971 (1986).
Buus, S., Sette, A., Colon, S. M., Jenis, D. M. & Grey, H. M. Cell 47, 1071–1077 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gotch, F., Rothbard, J., Howland, K. et al. Cytotoxic T lymphocytes recognize a fragment of influenza virus matrix protein in association with HLA-A2. Nature 326, 881–882 (1987). https://doi.org/10.1038/326881a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/326881a0
- Springer Nature Limited
This article is cited by
-
Newborn and child-like molecular signatures in older adults stem from TCR shifts across human lifespan
Nature Immunology (2023)
-
Investigation of CD4 and CD8 T cell-mediated protection against influenza A virus in a cohort study
BMC Medicine (2022)
-
Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope
Nature Structural & Molecular Biology (2017)
-
A heat-inactivated H7N3 vaccine induces cross-reactive cellular immunity in HLA-A2.1 transgenic mice
Virology Journal (2016)
-
Differential effect of HLA class-I versus class-II transgenes on human T and B cell reconstitution and function in NRG mice
Scientific Reports (2016)