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A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter

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Abstract

Adenovirus E1A proteins stimulate transcription by RNA polymerases II and III from many promoters1. The detailed mechanism of transcriptional activation (transactivation) by E1A proteins remains unclear, but genetic and biochemical results suggest that E1A products might act to stimulate the activity of cellular transcription factors1. In this study, a detailed mutational analysis of the adenovirus E1B promoter was undertaken to define the DNA sequences required for proper basal transcription and E1A transactivation. Two key findings emerged: first the E1B promoter is an unusually simple RNA polymerase II promoter requiring only two sequence elements for proper regulation, the TATA box2 and a binding site for transcription factor Sp13,4; and second only mutations in the TATA box interfere with E1A-transactivat ion, suggesting that E1A mediates its effect on this promoter through the TATA-box transcription factor.

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Authors and Affiliations

  1. Molecular Biology Institute, Department of Medicine, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California, 90024, USA

    Lily Wu, Martin C. Schmidt & Arnold Berk

  2. Division of Cardiology, Department of Medicine, Center for the Health Sciences, University of California at Los Angeles, Los Angeles, California, 90024, USA

    Debra S. E. Rosser

Authors
  1. Lily Wu
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  2. Debra S. E. Rosser
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  3. Martin C. Schmidt
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  4. Arnold Berk
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Wu, L., Rosser, D., Schmidt, M. et al. A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter. Nature 326, 512–515 (1987). https://doi.org/10.1038/326512a0

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